Development of blood collection tubes for glucose measurement using adenosine 3-phosphate and sodium fluoride as glycolytic inhibitors.

IF 2.1 4区医学 Q3 MEDICAL LABORATORY TECHNOLOGY Annals of Clinical Biochemistry Pub Date : 2024-03-01 Epub Date: 2023-08-30 DOI:10.1177/00045632231194829

Yukio Kume, Yuji Hirowatari, Makoto Kurano, Yutaka Yatomi, Makoto Matsushita

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Abstract

Background: Blood collection tubes with sodium fluoride (NaF) added as a glycolytic inhibitor are widely used for glucose measurement. However, the glycolytic inhibitory effects of NaF are insufficient, and decreases in glucose levels over time after blood collection have become a problem.

Methods: Blood from a volunteer collected using an NaF tube was used to compare the glycolysis inhibitory abilities of ATP and ADP. Blood samples from 10 volunteers were collected in NaF tubes and NaF tubes with added ATP (NaF-ATP tubes). The stability of glucose and haemoglobin (Hb)A1c after whole-blood storage from immediately after blood collection to 24 h later was compared.

Results: ATP and ADP had similar inhibitory effects on glycolysis, but ATP was selected as an additive for blood collection tubes because ADP was more haemolytic than ATP. We verified the ability of NaF blood collection tubes supplemented with ATP to inhibit glycolysis. Mean (± standard deviation) glucose levels (n=10) after storage for 24 h after blood collection decreased to -9.0 ± 2.7 mg/dL (-0.50 ± 0.15 mmol/L) in conventional NaF tubes. NaF-ATP(20) tubes with 20 mg (0.036 mmol) ATP added showed a reduced decrease, with a mean of -5.8 ± 2.9 mg/dL (-0.32 ± 0.16 mmol/L). NaF-ATP tubes also had no effect on HbA1c measurement.

Conclusion: This study reports on a blood collection tube that enables the measurement of glucose and HbA1c. Based on the results of validation, we conclude that NaF-ATP tubes can reduce decreases in glucose over time in stored whole blood compared to conventional NaF tubes.

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利用 3-磷酸腺苷和氟化钠作为糖酵解抑制剂，开发用于葡萄糖测量的采血管。

背景：添加了氟化钠（NaF）作为糖酵解抑制剂的采血管被广泛用于葡萄糖测量。然而，NaF 的糖酵解抑制作用并不充分，采血后血糖水平随时间推移而下降已成为一个问题：方法：使用 NaF 管采集一名志愿者的血液，比较 ATP 和 ADP 的糖酵解抑制能力。用 NaF 管和添加了 ATP 的 NaF 管（NaF-ATP 管）采集了 10 名志愿者的血液样本。结果显示，ATP 和 ADP 对血糖和血红蛋白（Hb）A1c 的抑制作用相似：结果：ATP 和 ADP 对糖酵解的抑制作用相似，但由于 ADP 的溶血作用强于 ATP，因此 ATP 被选为采血管的添加剂。我们验证了添加 ATP 的 NaF 采血管抑制糖酵解的能力。在传统的 NaF 采血管中，采血后储存 24 小时后的平均（± 标准偏差）葡萄糖水平（n=10）降至 -9.0 ± 2.7 mg/dL（-0.50 ± 0.15 mmol/L）。加入 20 毫克（0.036 毫摩尔）ATP 的 NaF-ATP(20) 管的下降幅度较小，平均为 -5.8 ± 2.9 毫克/分升（-0.32 ± 0.16 毫摩尔/升）。NaF-ATP 管对 HbA1c 测量也没有影响：本研究报告了一种可测量葡萄糖和 HbA1c 的采血管。根据验证结果，我们得出结论：与传统的 NaF 管相比，NaF-ATP 管可减少储存全血中葡萄糖随时间推移而降低的情况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Clinical Biochemistry Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

5.20

自引率

4.50%

发文量

期刊介绍： Annals of Clinical Biochemistry is the fully peer reviewed international journal of the Association for Clinical Biochemistry and Laboratory Medicine. Annals of Clinical Biochemistry accepts papers that contribute to knowledge in all fields of laboratory medicine, especially those pertaining to the understanding, diagnosis and treatment of human disease. It publishes papers on clinical biochemistry, clinical audit, metabolic medicine, immunology, genetics, biotechnology, haematology, microbiology, computing and management where they have both biochemical and clinical relevance. Papers describing evaluation or implementation of commercial reagent kits or the performance of new analysers require substantial original information. Unless of exceptional interest and novelty, studies dealing with the redox status in various diseases are not generally considered within the journal''s scope. Studies documenting the association of single nucleotide polymorphisms (SNPs) with particular phenotypes will not normally be considered, given the greater strength of genome wide association studies (GWAS). Research undertaken in non-human animals will not be considered for publication in the Annals. Annals of Clinical Biochemistry is also the official journal of NVKC (de Nederlandse Vereniging voor Klinische Chemie) and JSCC (Japan Society of Clinical Chemistry).