The Effects of Nebulized Inhaled Triptolide on Airway Inflammation in a Mouse Model of Asthma.

IF 2.1 4区 医学 Q3 RESPIRATORY SYSTEM Canadian respiratory journal Pub Date : 2023-01-01 DOI:10.1155/2023/2983092
Yafang Miao, Li Wei, Hao Chen, Zeming Zhang, Li Han
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Abstract

Inhalation of nebulized TP has received little attention in the past. Here, we intend to investigate the effect of nebulized inhaled TP on airway inflammation in a mouse model of asthma. 29 SPF BALB/c mice were divided into four groups: blank control (Blk, n = 5), normal saline (NS, n = 8), dexamethasone (Dex, n = 8), and TP (n = 8). During the process of sensitization, mice in the three intervention groups were treated with nebulized NS, an injection of Dex, and nebulized triptolide, respectively. Then bronchoalveolar lavage fluid (BALF), peripheral blood, and lung tissue were collected. Relevant cytokines, transcriptional factors, and CD4+Th17+ T cell proportions were assessed and compared. IL-6, IL-17, IL-23, and TGF-β1 demonstrated a significant difference between groups in the following order: Dex < TP < NS (P ≤ 0.001), while IL-10 changed in the opposite direction (P < 0.001). At the transcriptional level in lung tissue, the Ct value of IL-17 in the Dex group was significantly higher than in the NS and TP groups (P < 0.001). Meanwhile, it was higher in the TP group than in the NS group (P < 0.001). The Ct value of RORγt demonstrated a significant difference among three groups in the following order: Dex > TP > NS (P < 0.001). An opposite trend of FoxP3 Ct value was revealed in the order: NS > TP > Dex. The proportion of CD4+Th17+ cells was 9.53 ± 2.74% in the NS group, 4.23 ± 2.26% in the Dex group, and 6.76 ± 2.99% in the TP group, which shows significant differences between the NS and Dex (P < 0.001) or NS and TP groups (P < 0.05). Inhalation of nebulized triptolide can play a role in suppressing airway inflammation with inflammatory cytokines and transcriptional factors reduced and CD4+Th17+ T cells dampened, also in a manner less than injected dexamethasone.

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雾化吸入雷公藤甲内酯对哮喘小鼠气道炎症的影响。
吸入雾化TP在过去很少受到关注。在此,我们打算研究雾化吸入TP对哮喘小鼠气道炎症的影响。29只SPF级BALB/c小鼠分为4组:空白对照组(Blk, n = 5)、生理盐水组(NS, n = 8)、地塞米松组(Dex, n = 8)、TP组(n = 8)。在致敏过程中,三个干预组小鼠分别给予雾化NS、注射右美托咪唑、雾化雷公藤甲素。然后采集支气管肺泡灌洗液(BALF)、外周血和肺组织。评估和比较相关的细胞因子、转录因子和CD4+Th17+ T细胞比例。IL-6、IL-17、IL-23、TGF-β1在组间差异有统计学意义的顺序为:Dex P≤0.001),IL-10在组间差异有统计学意义(P < 0.001)。在肺组织转录水平上,Dex组IL-17的Ct值显著高于NS组和TP组(P < 0.001)。TP组明显高于NS组(P < 0.001)。rr γt的Ct值在三组间的差异依次为:Dex > TP > NS (P < 0.001)。FoxP3 Ct值的变化趋势为:NS > TP > Dex。NS组CD4+Th17+细胞比例为9.53±2.74%,Dex组为4.23±2.26%,TP组为6.76±2.99%,NS组与Dex组差异有统计学意义(P < 0.001), NS组与TP组差异有统计学意义(P < 0.05)。雾化吸入雷公藤甲素可起到抑制气道炎症的作用,炎症因子和转录因子减少,CD4+Th17+ T细胞受到抑制,其作用程度也低于注射地塞米松。
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来源期刊
Canadian respiratory journal
Canadian respiratory journal 医学-呼吸系统
CiteScore
4.20
自引率
0.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Canadian Respiratory Journal is a peer-reviewed, Open Access journal that aims to provide a multidisciplinary forum for research in all areas of respiratory medicine. The journal publishes original research articles, review articles, and clinical studies related to asthma, allergy, COPD, non-invasive ventilation, therapeutic intervention, lung cancer, airway and lung infections, as well as any other respiratory diseases.
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