Importance of Toxin Genes and Polymerase Chain Reaction-Based Open Reading Frame Type Analyses for Severe Staphylococcus aureus Infection in Children.

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES Japanese journal of infectious diseases Pub Date : 2023-11-22 Epub Date: 2023-08-31 DOI:10.7883/yoken.JJID.2023.194
Noriko Takeuchi, Naruhiko Ishiwada
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Abstract

This study analyzed 26 Staphylococcus aureus strains, including 16 methicillin-resistant S. aureus (MRSA) and 10 methicillin-susceptible S. aureus (MSSA), collected from eight medical institutions in the Chiba Prefecture that requested a toxin gene analysis between 2015 and 2021. A total of 14 Panton-Valentine leukocidin (PVL) positive strains were identified, including MSSA. PVL-positive strains were classified into seven types according to polymerase chain reaction-based open reading frame typing (POT); of these types, three POT MRSA strains have not been previously reported, and one has been previously reported as PVL-negative. Some strains tested positive for both PVL and toxic shock syndrome toxin 1. One POT type was identified in both PVL-positive and PVL-negative strains. To the best of our knowledge, this is the first report on the regional spread of highly pathogenic S. aureus strains based on the POT method in children from multiple medical institutions. This method is useful for estimating the spread of toxin gene-carrying strains in the community owing to its association with toxin genes. As the number of PVL-positive strains in Japan increases, it is important to analyze the isolates of severe S. aureus infections in children by combining toxin gene analyses with the POT method.

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毒素基因和基于聚合酶链反应的开放阅读框型分析对儿童重症金黄色葡萄球菌感染的重要性。
本研究分析了2015年至2021年间从千叶县8家医疗机构收集的26株金黄色葡萄球菌,其中包括16株耐甲氧西林金黄色葡萄球菌(MRSA)和10株甲氧西林敏感金黄色葡萄球菌(MSSA),这些机构要求进行毒素基因分析。共检出Panton-Valentine leukocidin (PVL)阳性菌株14株,包括MSSA。根据基于聚合酶链反应的开放阅读框分型(POT)将pvl阳性菌株分为7种类型;在这些类型中,有三种POT MRSA菌株以前没有报道过,其中一种以前报道过pvl阴性。部分菌株PVL和中毒性休克综合征毒素1均呈阳性。pvl阳性株和pvl阴性株均鉴定出一种POT型。据我们所知,这是第一份基于POT方法在多家医疗机构儿童中高致病性金黄色葡萄球菌菌株区域传播的报告。由于该方法与毒素基因相关,因此可用于估计毒素基因携带菌株在群落中的传播。随着日本pvl阳性菌株数量的增加,将毒素基因分析与POT方法相结合对儿童严重金黄色葡萄球菌感染分离株进行分析具有重要意义。
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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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