Amelioration effects of the soybean lecithin-gallic acid complex on iron-overload-induced oxidative stress and liver damage in C57BL/6J mice.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2023-12-01 DOI:10.1080/13880209.2022.2151632

Caihong Wu, Wenxin Zhang, Feifei Yan, Wenwen Dai, Fang Fang, Yanli Gao, Weiwei Cui

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引用次数: 3

Abstract

Context: Gallic acid (GA) and lecithin showed important roles in antioxidant and drug delivery, respectively. A complex synthesized from GA and soybean lecithin (SL-GAC), significantly improved bioavailability of GA and pharmacological activities. However, the antioxidant activity of SL-GAC and its effect on iron-overload-induced liver injury remains unexplored.

Objective: This study investigates the antioxidant properties of SL-GAC in vitro and in mice, and its remediating effects against liver injury by iron-overloaded.

Materials and methods: In vitro, free radical scavenging activity, lipid peroxidation inhibition, and ferric reducing power of SL-GAC were measured by absorbance photometry. In vivo, C57BL/6J mice were randomized into 4 groups: control, iron-overloaded, iron-overloaded + deferoxamine, and iron-overloaded + SL-GAC. Treatments with deferoxamine (150 mg/kg/intraperitioneally) and SL-GAC (200 mg/kg/orally) were given to the desired groups for 12 weeks, daily. Iron levels, oxidative stress, and biochemical parameters were determined by histopathological examination and molecular biological techniques.

Results: In vitro, SL-GAC showed DPPH and ABTS free radicals scavenging activity with IC₅₀ values equal to 24.92 and 128.36 μg/mL, respectively. In C57BL/6J mice, SL-GAC significantly reduced the levels of serum iron (22.82%), liver iron (50.29%), aspartate transaminase (25.97%), alanine transaminase (38.07%), gamma glutamyl transferase (42.11%), malondialdehyde (19.82%), total cholesterol (45.96%), triglyceride (34.90%), ferritin light chain (18.51%) and transferrin receptor (27.39%), while up-regulated the levels of superoxide dismutase (24.69%), and glutathione (11.91%).

Conclusions: These findings encourage the use of SL-GAC to treat liver injury induced by iron-overloaded. Further in vivo and in vitro studies are needed to validate its potential in clinical medicine.

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大豆卵磷脂-没食子酸复合物对铁超载诱导的C57BL/6J小鼠氧化应激和肝损伤的改善作用。

背景：没食子酸（GA）和卵磷脂分别在抗氧化和给药方面发挥着重要作用。由GA和大豆卵磷脂合成的复合物（SL-GAC），显著提高了GA的生物利用度和药理活性。然而，SL-GAC的抗氧化活性及其对铁过载诱导的肝损伤的影响仍有待探索。目的：研究SL-GAC在体外和小鼠体内的抗氧化性能及其对铁超载肝损伤的修复作用。材料和方法：采用吸光度法测定SL-GAC的自由基清除活性、脂质过氧化抑制作用和铁还原能力。在体内，C57BL/6J小鼠被随机分为4组：对照组、铁过载组、铁超载组 + 去铁胺和铁过载 + SL-GAC。去铁胺治疗（150 mg/kg腹腔注射）和SL-GAC（200 mg/kg/口服）给所需组12 周，每天。通过组织病理学检查和分子生物学技术测定铁水平、氧化应激和生化参数。结果：SL-GAC在体外对DPPH和ABTS自由基具有清除活性，IC50分别为24.92和128.36 μg/mL。在C57BL/6J小鼠中，SL-GAC显著降低血清铁（22.82%）、肝铁（50.29%）、天冬氨酸转氨酶（25.97%）、丙氨酸转氨酶（38.07%）、γ-谷氨酰转移酶（42.11%）、丙二醛（19.82%）、总胆固醇（45.96%）、甘油三酯（34.90%）、铁蛋白轻链（18.51%）和转铁蛋白受体（27.39%）的水平，同时上调超氧化物歧化酶（24.69%）和谷胱甘肽（11.91%）的水平。需要进一步的体内和体外研究来验证其在临床医学中的潜力。

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