Importance of miR-UL-148D Expression Pattern in Cytomegalovirus Infected Transplant Patients.

IF 0.3 Q4 TRANSPLANTATION International Journal of Organ Transplantation Medicine Pub Date : 2021-01-01

M Golshan, R Yaghobi, B Geramizadeh, A Afshari, J Roozbeh, S A Malek-Hosseini

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引用次数: 0

Abstract

Background: MicroRNAs (miRNAs) are endogenous, 18-22 nucleotide non-coding RNA molecules. Human cytomegalovirus (HCMV) is a ubiquitous and particular herpes virus that encodes miRNAs, which increases gradually in the presence of infection. One of the important viral miRNAs is HCMV-miRUL-148D, which plays a role in establishing and maintaining viral latency.

Objective: The current study aimed to evaluate the expression levels of HCMV-miRUL-148D in active and inactive HCMV infected transplant patient groups compared to healthy individuals.

Methods: Total RNA was extracted from blood samples of 60 solid organ transplant patients and 30healthy controls. In-house SYBR Green Real-Time PCR evaluated the expression levels of studied miRNAand gene.

Results: The expression level of the UL-148D gene was significantly higher in the active HCMV infectedpatients (p=0.001) compared to other groups. While the miRUL-148D expression level significantly increased in the inactive HCMV-infected patients (p<0.001) compared to other groups.

Conclusion: Increased miRUL-148D expression level in the inactive HCMV-infected transplant patients indicates the potential role of this miRUL-148D as a biomarker of the HCMV latent stage.

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巨细胞病毒感染移植患者体内 miR-UL-148D 表达模式的重要性

背景：微小RNA（miRNA）是一种内源性、18-22个核苷酸的非编码RNA分子。人类巨细胞病毒（HCMV）是一种无处不在的特殊疱疹病毒，它编码的 miRNAs 在感染时会逐渐增加。其中一个重要的病毒 miRNA 是 HCMV-miRUL-148D，它在建立和维持病毒潜伏期方面发挥作用：本研究旨在评估活动性和非活动性 HCMV 感染移植患者群体与健康人相比的 HCMV-miRUL-148D 表达水平：方法：从 60 名实体器官移植患者和 30 名健康对照者的血液样本中提取总 RNA。方法：从 60 名实体器官移植患者和 30 名健康对照者的血液样本中提取总 RNA，并在内部用 SYBR Green Real-Time PCR 评估所研究的 miRNA 和基因的表达水平：结果：与其他组别相比，活动性 HCMV 感染者的 UL-148D 基因表达水平明显更高（p=0.001）。而在非活动性 HCMV 感染者中，miRUL-148D 的表达水平明显升高（P=0.001）：在非活动性 HCMV 感染移植患者中，miRUL-148D 表达水平的升高表明，miRUL-148D 有可能成为 HCMV 潜伏期的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Organ Transplantation Medicine TRANSPLANTATION-

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： The International Journal of Organ Transplantation Medicine (IJOTM) is a quarterly peer-reviewed English-language journal that publishes high-quality basic sciences and clinical research on transplantation. The scope of the journal includes organ and tissue donation, procurement and preservation; surgical techniques, innovations, and novelties in all aspects of transplantation; genomics and immunobiology; immunosuppressive drugs and pharmacology relevant to transplantation; graft survival and prevention of graft dysfunction and failure; clinical trials and population analyses in the field of transplantation; transplant complications; cell and tissue transplantation; infection; post-transplant malignancies; sociological and ethical issues and xenotransplantation.