Kisspeptin decreases the adverse effects of human ovarian vitrification by regulating ROS-related apoptotic occurrences.

IF 1.5 4区 生物学 Q4 CELL BIOLOGY Zygote Pub Date : 2023-12-01 Epub Date: 2023-09-01 DOI:10.1017/S0967199423000412
Anahita Tavakoli, Fereshteh Aliakbari, Malek Soleimani Mehranjani
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Abstract

Kisspeptin is characterized as a neuropeptide with a pivotal function in female and male infertility, and its antioxidant properties have been demonstrated. In this study, the effects of kisspeptin on the improvement of the vitrification and thawing results of human ovarian tissues were investigated. In this work, 12 ovaries from patients who underwent hysterectomy were collected laparoscopically, and then 32 samples from each of their tissues were taken. Haematoxylin and eosin (H&E) staining was performed to check the normality of the ovarian tissue and, subsequently, the samples were allocated randomly into four groups, including: (1) fresh (control), (2) vitrification, (3) vitrified + 1 μM kisspeptin, and (4) vitrified + 10 μM kisspeptin groups. After vitrification, thawing, and tissue culture processes, H&E staining for tissue quality assessment, terminal deoxynucleotidyl transferase dUTP nick end labelling assay for apoptosis evaluation, and malondialdehyde (MDA), superoxide dismutase (SOD), and ferric reducing ability of plasma tests for oxidative stress appraisal were carried out. Our histological results showed incoherency of ovarian tissue morphology in the vitrification group compared with other groups. Other findings implicated increased apoptosis rate and MDA concentration and reduced SOD activity and total antioxidant capacity (TAC) in the vitrification group compared with the control group (P < 0.05). Moreover, decreased apoptosis rate and MDA concentration, and increased TAC and SOD function were observed in the vitrification with kisspeptin groups (1 μM and 10 μM) compared with the vitrified group (P < 0.05). Our reports express that kisspeptin is an effective agent to overcome the negative effects of vitrification by regulating reactive oxygen species-related apoptotic processes.

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Kisspeptin通过调节ros相关的凋亡事件减少人卵巢玻璃化的不良反应。
Kisspeptin是一种神经肽,在女性和男性不育中具有关键功能,其抗氧化特性已被证明。本研究探讨了kisspeptin对改善人卵巢组织玻璃化和解冻效果的影响。在这项工作中,通过腹腔镜收集了子宫切除术患者的12个卵巢,然后从每个组织中提取了32个样本。采用血红素和伊红(H&E)染色检查卵巢组织是否正常,然后将样本随机分为4组,包括:(1)新鲜(对照),(2)玻璃化,(3)玻璃化+ 1 μM kisspeptin组,(4)玻璃化+ 10 μM kisspeptin组。经过玻璃化、解冻和组织培养后,进行H&E染色评价组织质量,末端脱氧核苷酸转移酶dUTP缺口末端标记试验评价细胞凋亡,血浆丙二醛(MDA)、超氧化物歧化酶(SOD)和铁还原能力试验评价氧化应激。我们的组织学结果显示,与其他组相比,玻璃化组卵巢组织形态不一致。与对照组相比,玻璃化组细胞凋亡率和MDA浓度升高,SOD活性和总抗氧化能力(TAC)降低(P < 0.05)。kisspeptin玻璃化组(1 μM和10 μM)与玻璃化组比较,细胞凋亡率和MDA浓度降低,TAC和SOD功能升高(P < 0.05)。我们的报告表明,kisspeptin是一种有效的药物,通过调节活性氧相关的凋亡过程来克服玻璃化的负面影响。
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来源期刊
Zygote
Zygote 生物-发育生物学
CiteScore
1.70
自引率
5.90%
发文量
117
审稿时长
6-12 weeks
期刊介绍: An international journal dedicated to the rapid publication of original research in early embryology, Zygote covers interdisciplinary studies on gametogenesis through fertilization to gastrulation in animals and humans. The scope has been expanded to include clinical papers, molecular and developmental genetics. The editors will favour work describing fundamental processes in the cellular and molecular mechanisms of animal development, and, in particular, the identification of unifying principles in biology. Nonetheless, new technologies, review articles, debates and letters will become a prominent feature.
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