Study on the mechanism of lupenone for treating type 2 diabetes by integrating pharmacological evaluation and network pharmacology.

IF 3.9 3区医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pharmaceutical Biology Pub Date : 2022-12-01 DOI:10.1080/13880209.2022.2067568

Feng Xu, Mei Zhang, Hongmei Wu, Yuanmin Wang, Ye Yang, Xiangpei Wang

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引用次数: 4

Abstract

Context: Lupenone (LUP) is the active ingredient of Musa basjoo Sieb. et Zucc. (Musaceae) with antidiabetes effects, but an unclear underlying mechanism of action.

Objective: Animal experiments combined with network pharmacology were used to explore the mechanism of LUP for treating diabetes.

Materials and methods: Insulin resistance (IR) in male Sprague-Dawley rats with type 2 diabetic was induced using a high-fat diet and streptozotocin. The selected rats were divided into normal group, model group, positive group and LUP (2.0, 4.0 and 8.0 mg/kg) groups, and orally administrated twice daily with Tween 80, rosiglitazone or LUP. Fasting blood glucose (FBG), oxidative stress index, blood lipids and IR-related targets were detected. A network pharmacology analysis was performed.

Results: Compared to the model group, LUP (8.0 mg/kg) significantly decreased the levels of FBG (22.3%), LEP (9.5%), HbA1c (14.9%) and MDA (12.3%), increased the ADPN (24.2%) levels and GSH-PX activity (12.4%) (p < 0.05), improved oxidative stress, lipid metabolism disorders and pancreas pathological changes, increased the mRNA and protein expression of InsR (3.7-fold and 1.3-fold), IRS-1 (3-fold and 2-fold), IRS-2 (2-fold and 1.6-fold), GLUT-4 (2-fold and 2.4-fold) in skeletal muscle and IRS-1 (6-fold and 1.6-fold), IRS-2 (5.8-fold and 1.5-fold), GLUT-4 (2.5-fold and 1.7-fold) and PPAR-γ (7-fold and 1.4-fold) in adipose tissue (p < 0.05). Network pharmacology analysis revealed that LUP improves IR by multiple targets and signal pathways.

Conclusions: The mechanism of LUP for treating diabetes is related to improving IR. LUP has the potential to be developed as a new drug for treating type 2 diabetes.

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结合药理评价和网络药理学研究lupenone治疗2型糖尿病的作用机制。

背景:Lupenone (LUP)是芭蕉属植物的活性成分。调查。具有抗糖尿病作用，但作用机制尚不清楚。目的:采用动物实验结合网络药理学的方法，探讨LUP治疗糖尿病的作用机制。材料与方法:采用高脂饮食和链脲佐菌素诱导雄性2型糖尿病大鼠胰岛素抵抗(IR)。将所选大鼠分为正常组、模型组、阳性组和LUP(2.0、4.0、8.0 mg/kg)组，每日2次口服Tween 80、罗格列酮或LUP。检测空腹血糖(FBG)、氧化应激指数、血脂及ir相关指标。进行网络药理学分析。结果:与模型组比较，LUP (8.0 mg/kg)显著降低FBG(22.3%)、LEP(9.5%)、HbA1c(14.9%)、MDA(12.3%)水平，提高ADPN(24.2%)水平和GSH-PX活性(12.4%)(p p) (p p)。LUP有潜力成为治疗2型糖尿病的新药。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmaceutical Biology 医学-药学

CiteScore

6.70

自引率

2.60%

发文量

191

审稿时长

1 months

期刊介绍： Pharmaceutical Biology will publish manuscripts describing the discovery, methods for discovery, description, analysis characterization, and production/isolation (including sources and surveys) of biologically-active chemicals or other substances, drugs, pharmaceutical products, or preparations utilized in systems of traditional medicine. Topics may generally encompass any facet of natural product research related to pharmaceutical biology. Papers dealing with agents or topics related to natural product drugs are also appropriate (e.g., semi-synthetic derivatives). Manuscripts will be published as reviews, perspectives, regular research articles, and short communications. The primary criteria for acceptance and publication are scientific rigor and potential to advance the field.