Antioxidant, cytotoxic, and genotoxic potentials of the gum of Ferula gummosa Boiss on PC-3 cells.

IF 1.9 Q3 CHEMISTRY, MEDICINAL Avicenna Journal of Phytomedicine Pub Date : 2023-05-01 DOI:10.22038/AJP.2022.21605
Fatemeh Eizadifard, Majid Tafrihi, Maryam Mohadjerani
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Abstract

Objective: Ferula gummosa Boiss is a well-known Iranian endemic plant that has been used in Iranian traditional medicine against various diseases. This study aimed to evaluate the antioxidant and cytotoxic capacity of F. gummosa gum on prostate cancer PC-3 cells.

Materials and methods: In this study, we evaluated the total phenolic and flavonoid contents, and antioxidant potentials of the gum. The MTT experiment was conducted to assess the cytotoxic potential of the gum on PC-3 cells. The clonogenic, micronucleus formation, and acridine orange/ethidium bromide staining methods were used to evaluate the survival and proliferation of PC-3 cells. DNA degradation and caspase 3/7 activity evaluations were used to assess apoptosis. The inhibitory effect on the migration of PC-3 cells was examined by in vitro wound-healing experiment.

Results: Total phenolic and flavonoid contents, and antioxidant potential of the gum were 9.22 mg of gallic acid equivalent (GAE)/g, 3.6 mg of quercetin equivalents (QE) /g of the extract, and 13 μg/ml, respectively (compared to gallic acid and quercetin, respectively) (p<0.05). The IC50 value was 9.14 µg/ml for 48 hours (compared to non-treated cells) (p<0.01). The pattern of DNA degradation, and caspase 3/7 activity levels (compared to non-treated cells) (p<0.05) proposed decreased cell viability that may be due to apoptosis induction. Microscopic observations revealed nuclear condensation, a significant increase in the formation of micronuclei, and inhibition of forming colonies (compared to non-treated cells) (p<0.01) in PC-3 cells treated with 8 and 10 μg/ml of the gum. Wound-healing assessment showed the migration suppression potentials of the gum (compared to non-treated cells) (p<0.05).

Conclusion: These results indicate that F. gummosa has considerable antioxidant and cytotoxic properties that can make it a good nominee for subsequent investigations.

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树胶对PC-3细胞的抗氧化、细胞毒性和遗传毒性潜力。
目的:软胶Ferula gummosa Boiss是伊朗著名的地方性植物,在伊朗传统医学中被用于治疗各种疾病。本研究旨在评价软胶对前列腺癌症PC-3细胞的抗氧化和细胞毒性。材料和方法:在本研究中,我们评估了口香糖的总酚和类黄酮含量以及抗氧化潜力。MTT实验评估了口香糖对PC-3细胞的细胞毒性潜力。采用克隆、微核形成和吖啶橙/溴化乙锭染色方法评价PC-3细胞的存活和增殖。DNA降解和胱天蛋白酶3/7活性评估用于评估细胞凋亡。通过体外伤口愈合实验检测了对PC-3细胞迁移的抑制作用。结果:该胶的总酚类和黄酮类含量以及抗氧化能力分别为9.22mg没食子酸当量(GAE)/g、3.6mg槲皮素当量(QE)/g提取物和13μg/ml,分别(与没食子酸和槲皮素相比)(p50值在48小时内为9.14µg/ml(与未处理的细胞相比)(P结论:这些结果表明,软糖具有相当大的抗氧化和细胞毒性特性,可以使其成为后续研究的良好候选物。
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来源期刊
Avicenna Journal of Phytomedicine
Avicenna Journal of Phytomedicine CHEMISTRY, MEDICINAL-
CiteScore
3.40
自引率
4.50%
发文量
17
审稿时长
6 weeks
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