Antiperspirant effects and mechanism investigation of Mulisan decoction in rats based on plasma metabolomics.

IF 3.9 3区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pharmaceutical Biology Pub Date : 2022-12-01 DOI:10.1080/13880209.2022.2074465
Shan-Peng Ma, Wei-Ping Ma, Shi-Ning Yin, Xiang-Yue Chen, Xiao-Qing Ma, Bao-Hong Wei, Jing-Guang Lu, Hong-Bing Liu
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Abstract

Context: Mulisan decoction (MLS) is a classic formula of traditional Chinese medicine for treating hyperhidrosis. The mechanism remains unclear.

Objective: To investigate the antiperspirant effect and underlying mechanisms of MLS.

Materials and methods: Fifty rats were divided into control, model, and three doses of MLS intervention groups (n = 10). Rats except for control group were induced diseases features of the applicable scope of MLS via i.p. reserpine (0.5 mg/kg/d) for 10 days. From day 11, MLS groups were administrated orally MLS at 0.6, 3, and 15 g/kg once a day for 14 days, respectively. After the last administration, sweating was induced in all rats via s.c. pilocarpine (25 mg/kg), the right hind foot of rats was stained, and sweat point numbers were observed. Rat serum was collected to detect IL-2, IL-6, IFN-γ, and TNF-α. Rat plasma was collected for endogenous metabolite analysis via UPLC-QE-Focus-MS.

Results: Rats treated with MLS presented a significant decrease in sweat point numbers (13.5%), increase in body weight (13.2%), and promotion in the balance of Th1/Th2 cytokine ratio via increasing IL-2 (38.3%), IFN-γ (20.1%), and TNF-α (22.0%) and decreasing IL-6 (24.7%) compared with the model group (p < 0.05). Plasma metabolomics disclosed 15 potential biomarkers related to model rats, of which two could be significantly reversed by MLS (p < 0.05). The involved pathways were pantothenate and CoA biosynthesis, and porphyrin metabolism.

Conclusions: MLS demonstrated a good antiperspirant effect and metabolism improvement. These findings inspire more clinical study validation on immune improvement and antiperspirant effect.

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基于血浆代谢组学的木散汤大鼠止汗作用及机制研究。
背景:木散汤是治疗多汗症的经典中药方剂。其机制尚不清楚。目的:探讨MLS的止汗作用及其机制。材料与方法:50只大鼠分为对照组、模型组和3个剂量MLS干预组(n = 10)。除对照组外,其余大鼠经利血平(0.5 mg/kg/d)灌胃10 d诱导MLS适用范围内的疾病特征。从第11天开始,MLS组分别以0.6、3、15 g/kg的剂量口服MLS,每天1次,连用14 d。末次给药后,所有大鼠经皮罗卡品(25 mg/kg)诱导排汗,对大鼠右后足进行染色,观察排汗点数。收集大鼠血清检测IL-2、IL-6、IFN-γ、TNF-α。采集大鼠血浆,采用uplc - q - focus - ms进行内源性代谢物分析。结果:与模型组相比,MLS治疗大鼠的汗点数显著减少(13.5%),体重显著增加(13.2%),通过IL-2(38.3%)、IFN-γ(20.1%)、TNF-α(22.0%)和IL-6(24.7%)的升高,促进Th1/Th2细胞因子比值平衡(p < 0.05)。结论:MLS具有良好的止汗作用和代谢改善作用。这些发现激发了更多关于免疫改善和止汗效果的临床研究验证。
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来源期刊
Pharmaceutical Biology
Pharmaceutical Biology 医学-药学
CiteScore
6.70
自引率
2.60%
发文量
191
审稿时长
1 months
期刊介绍: Pharmaceutical Biology will publish manuscripts describing the discovery, methods for discovery, description, analysis characterization, and production/isolation (including sources and surveys) of biologically-active chemicals or other substances, drugs, pharmaceutical products, or preparations utilized in systems of traditional medicine. Topics may generally encompass any facet of natural product research related to pharmaceutical biology. Papers dealing with agents or topics related to natural product drugs are also appropriate (e.g., semi-synthetic derivatives). Manuscripts will be published as reviews, perspectives, regular research articles, and short communications. The primary criteria for acceptance and publication are scientific rigor and potential to advance the field.
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