Antidepressant effects of acupuncture in a murine model: regulation of neurotrophic factors.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-02-01 DOI:10.1177/09645284221085279

Teruhisa Yamamoto, Jun Kawanokuchi, Nobuyuki Nagaoka, Ken Takagi, Torao Ishida, Tomoya Hayashi, Ning Ma

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引用次数: 2

Abstract

Background: GV20 and Yintang are important targets in acupuncture treatment for depression. In this study, we examined the antidepressant effects of simultaneous acupuncture stimulation at GV20 and Yintang.

Methods: We compared the antidepressant effects of manual acupuncture (MA) stimulation at GV20 and Yintang, compared to acupuncture stimulation at two control point locations on the back of the mice (overlying the spinal column) and imipramine administration in a forced swimming (FS)-induced mouse model of depression, and examined the mRNA and protein expression of neurotrophic factors, including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin (NT)-3, and NT-4/5 in the brains by real-time polymerase chain reaction in two different experimental schedules - preventive (MA given alongside FS modelling) and therapeutic (MA given after FS-induced depression was already established).

Results: MA at GV20 and Yintang significantly reduced the immobility time of mice with FS-induced depression in both preventive and therapeutic experimental designs, with effects that were comparable to those of imipramine administration. Immobility time following simultaneous acupuncture stimulation of the two control point locations overlying the spinal column was significantly suppressed only 2 weeks after the start of FS in the preventive effect experiment, and the suppressive effect was significantly lower than that of simultaneous acupuncture stimulation at GV20 and Yintang. In the therapeutic effect experiment, there was no change in the increase in immobility time after the end of FS. MA at GV20 and Yintang significantly increased the expression of BDNF and NT-3 in the preventive evaluation and NGF, BDNF, NT-3, and NT-4/5 in the therapeutic effect evaluation.

Conclusion: Our findings suggest that simultaneous acupuncture stimulation at GV20 and Yintang is effective for the prevention and treatment of depression, and the effect likely involves modulation of the expression of multiple neurotrophic factors.

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针刺在小鼠模型中的抗抑郁作用:神经营养因子的调节。

背景:GV20和印堂是针刺治疗抑郁症的重要靶点。在本研究中，我们观察了同时针刺刺激GV20和印堂的抗抑郁作用。方法:在强迫游泳(FS)诱导的小鼠抑郁模型中，我们比较了手针(MA)刺激GV20和荫塘的抗抑郁作用，以及针刺刺激小鼠背部(脊柱上)的两个控制点位置和丙咪丙胺的抗抑郁作用，并检测了神经营养因子mRNA和蛋白的表达，包括神经生长因子(NGF)、脑源性神经营养因子(BDNF)、神经营养因子(NT)-3、在两种不同的实验计划下——预防性(与FS建模同时给予MA)和治疗性(在FS诱导的抑郁已经建立后给予MA)，通过实时聚合酶链反应检测大脑中NT-4/5的含量。结果:在预防和治疗实验设计中，MA在GV20和茵堂均可显著减少fs诱导的抑郁症小鼠的静止时间，其作用与丙咪嗪给药相当。在预防效果实验中，同时针灸刺激脊柱上两个控制点的静止时间仅在FS开始后2周就被显著抑制，且抑制作用明显低于同时针灸刺激GV20和印堂。在治疗效果实验中，FS结束后静止时间的增加没有变化。GV20和印堂的MA在预防评估中显著增加BDNF和NT-3的表达，在治疗效果评估中显著增加NGF、BDNF、NT-3和NT-4/5的表达。结论:针刺同时刺激GV20和印堂可有效预防和治疗抑郁症，其作用可能与调节多种神经营养因子的表达有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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