Effect of formalin fixation on tumour size and margins in head and neck cancer specimens.

IF 2.1 4区医学 Q2 OTORHINOLARYNGOLOGY Acta Otorhinolaryngologica Italica Pub Date : 2022-10-01 DOI:10.14639/0392-100X-N2185

Kshithi K, Vikrant Kamboj, Suja Sreedharan, Vijendra Shenoy S, Thripthi Rai, Sushmitha Kabekkodu

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引用次数: 1

Abstract

Objectives: To quantify tumour size and margin shrinkage due to formalin fixation in head and neck cancer specimens and determine its effect on tumour staging and margin clearance.

Methods: Tumour specimens were measured immediate post-resection and at 24 hours after fixation. Tumour was measured in 2 dimensions and one margin was measured. Shrinkage was categorised as < 10%, 10-20%, and > 20%. Effect of shrinkage on tumour stage and margin clearance were evaluated.

Results: A total of 50 specimens were analysed. The tumour AP (Anterior Posterior) and SI (Superior Inferior) measurements showed significant shrinkage with a mean difference of 22.93% and 21.69% respectively. > 20% shrinkage was noted in 78% of AP and 58% of SI measurements. Margins showed a mean difference of 25.61%. 84% of margins demonstrated > 20% shrinkage. In all, 46.7% of T3 and 23% of T2 tumours pre-fixation were downstaged to T2 and T1, respectively, post-fixation.

Conclusions: Formalin fixation alone can be responsible for significant shrinkage of tumour and margin dimensions in head and neck specimens. It is suggested that decisions regarding the treatment plan should be made on clinical staging of primary tumour rather than pathological staging. In addition, post-excision pre-fixation margins should be considered for treatment planning.

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福尔马林固定对头颈癌标本肿瘤大小和边缘的影响。

目的:量化头颈部肿瘤标本中福尔马林固定引起的肿瘤大小和边缘缩小，并确定其对肿瘤分期和边缘清除的影响。方法:肿瘤标本在切除后立即和固定后24小时测量。二维测量肿瘤，并测量一个边缘。收缩率分为< 10%、10-20%和> 20%。评估收缩对肿瘤分期和边缘清除率的影响。结果:共分析标本50例。肿瘤AP(前后)和SI(上下)测量显示显著缩小，平均差异分别为22.93%和21.69%。在78%的AP和58%的SI测量中发现了> 20%的收缩。边际平均差异为25.61%。84%的边缘显示> 20%的收缩。总的来说，46.7%的T3和23%的T2肿瘤在固定后分别下降到T2和T1。结论:单独福尔马林固定可导致头颈部标本肿瘤和边缘尺寸显著缩小。建议根据原发肿瘤的临床分期而不是病理分期来决定治疗方案。此外，在制定治疗计划时应考虑切除后的预固定边缘。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Otorhinolaryngologica Italica OTORHINOLARYNGOLOGY-

CiteScore

3.40

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Acta Otorhinolaryngologica Italica first appeared as “Annali di Laringologia Otologia e Faringologia” and was founded in 1901 by Giulio Masini. It is the official publication of the Italian Hospital Otology Association (A.O.O.I.) and, since 1976, also of the Società Italiana di Otorinolaringoiatria e Chirurgia Cervico-Facciale (S.I.O.Ch.C.-F.). The journal publishes original articles (clinical trials, cohort studies, case-control studies, cross-sectional surveys, and diagnostic test assessments) of interest in the field of otorhinolaryngology as well as clinical techniques and technology (a short report of unique or original methods for surgical techniques, medical management or new devices or technology), editorials (including editorial guests – special contribution) and letters to the Editor-in-Chief. Articles concerning science investigations and well prepared systematic reviews (including meta-analyses) on themes related to basic science, clinical otorhinolaryngology and head and neck surgery have high priority.