Three-dimensional visualization of human brain tumors using the CUBIC technique.

IF 2.7 3区医学 Q2 CLINICAL NEUROLOGY Brain Tumor Pathology Pub Date : 2023-01-01 DOI:10.1007/s10014-022-00445-2

Yangyang Xu, Qi He, Mengqi Wang, Yang Wu, Yifeng Shi, Wei Wang, Jie Zhang

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引用次数: 1

Abstract

Application of tissue clearing techniques on human brain tumors is still limited. This study was to investigate the application of CUBIC on 3D pathological studies of human brain tumors. Brain tumor specimens derived from 21 patients were cleared with CUBIC. Immunostaining was conducted on cleared specimens to label astrocytes, microglia and microvessels, respectively. All tumor specimens achieved transparency after clearing. Immunostaining and CUBIC are well compatible in a variety of human brain tumors. Spatial morphologies of microvessels, astrocytes and microglia of tumors were clearly visualized in 3D, and their 3D morphological parameters were easily quantified. By comparing the quantitative morphological parameters of microvessels among brain tumors of different malignancy, we found that mean vascular diameter was positively correlated with tumor malignancy. Our study demonstrates that CUBIC can be successfully applied to 3D pathological studies of various human brain tumors, and 3D studies of human brain tumors hold great promise in helping us better understand brain tumor pathology in the future.

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使用CUBIC技术的人类脑肿瘤的三维可视化。

组织清除技术在人类脑肿瘤中的应用仍然有限。本研究旨在探讨CUBIC在人脑肿瘤三维病理研究中的应用。21例患者的脑肿瘤标本用CUBIC清除。对清除后的标本进行免疫染色，分别标记星形胶质细胞、小胶质细胞和微血管。所有肿瘤标本清除后均透明。免疫染色和CUBIC在多种人类脑肿瘤中具有良好的相容性。肿瘤微血管、星形胶质细胞和小胶质细胞的三维空间形态清晰可见，其三维形态参数易于量化。通过比较不同恶性脑肿瘤微血管的定量形态学参数，我们发现平均血管直径与肿瘤恶性程度呈正相关。我们的研究表明，CUBIC可以成功地应用于各种人类脑肿瘤的三维病理研究，人类脑肿瘤的三维研究在未来帮助我们更好地了解脑肿瘤病理方面具有很大的前景。

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来源期刊

Brain Tumor Pathology 医学-病理学

CiteScore

5.40

自引率

9.10%

发文量

审稿时长

>12 weeks

期刊介绍： Brain Tumor Pathology is the official journal of the Japan Society of Brain Tumor Pathology. This international journal documents the latest research and topical debate in all clinical and experimental fields relating to brain tumors, especially brain tumor pathology. The journal has been published since 1983 and has been recognized worldwide as a unique journal of high quality. The journal welcomes the submission of manuscripts from any country. Membership in the society is not a prerequisite for submission. The journal publishes original articles, case reports, rapid short communications, instructional lectures, review articles, letters to the editor, and topics.Review articles and Topics may be recommended at the annual meeting of the Japan Society of Brain Tumor Pathology. All contributions should be aimed at promoting international scientific collaboration.