Clinacanthus nutans L Extracts Reduce the Serum Tumor Necrosis Factor-α, Malondialdehyde, and Interleukin-6 Levels and Improve the Langerhans Islet Area in Diabetic Rat Models.

IF 2.7 Q3 ENDOCRINOLOGY & METABOLISM Clinical Medicine Insights-Endocrinology and Diabetes Pub Date : 2023-01-01 DOI:10.1177/11795514231196462

Arifa Mustika, Nurmawati Fatimah, Indri Safitri, Nurlaili Susanti, Nurul Shahfiza Noor

{"title":"Clinacanthus nutans L Extracts Reduce the Serum Tumor Necrosis Factor-α, Malondialdehyde, and Interleukin-6 Levels and Improve the Langerhans Islet Area in Diabetic Rat Models.","authors":"Arifa Mustika, Nurmawati Fatimah, Indri Safitri, Nurlaili Susanti, Nurul Shahfiza Noor","doi":"10.1177/11795514231196462","DOIUrl":null,"url":null,"abstract":"Background: Diabetes mellitus-induced hyperglycemia increases oxidative stress and inflammatory cytokine production, which play a significant role in the damage and apoptosis of pancreatic β cells. Therefore, the administration of medications that can reduce oxidative stress and inflammation plays an important role in diabetes treatment.Objective: To probe the Clinacanthus nutans leaf extract effect on oxidative stress and inflammatory markers and the Langerhans islet area in diabetic rat models.Design: An experimental laboratory in the animal model.Methods: Twenty-five diabetic rat models were randomly assigned into 5 clusters. Clusters 1, 2, and 3 were administered with C. nutans leaf extract in aqueous suspension with vehicle 1% Na-CMC at 75 mg/kg body weight (BW), 150 mg/kg BW, and 300 mg/kg BW, respectively. Cluster 4 was diabetic control rats administered with metformin at a 21 mg/rat dose. Cluster 5 was a control diabetic rat only administered with 1% Na-CMC suspension. Treatment was administered orally for 14 days. On the 15th day, the rats were sacrificed to obtain blood samples and pancreatic tissues. Serum interleukin (IL)-6, malondialdehyde (MDA), and tumor necrosis factor (TNF-α) were measured using the enzyme-linked immunosorbent assay (ELISA) method. Histopathological examination was performed by counting the Langerhans islet areas.Results: The average IL-6, MDA, and TNF-α levels declined in the cluster receiving C. nutans extract and were significantly different from the untreated cluster (P < .05). Histopathological examination revealed a significant upsurge in the Langerhans islets area in diabetic rats receiving C. nutans extract at doses of 75 and 150 mg/kg (P < .05).Conclusion: C. nutans leaf extract reduced the serum MDA, TNF-α, and IL-6 levels, and increased the Langerhans islets area in a diabetic rat model.","PeriodicalId":44715,"journal":{"name":"Clinical Medicine Insights-Endocrinology and Diabetes","volume":"16 ","pages":"11795514231196462"},"PeriodicalIF":2.7000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/ef/10.1177_11795514231196462.PMC10492484.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Medicine Insights-Endocrinology and Diabetes","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/11795514231196462","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Diabetes mellitus-induced hyperglycemia increases oxidative stress and inflammatory cytokine production, which play a significant role in the damage and apoptosis of pancreatic β cells. Therefore, the administration of medications that can reduce oxidative stress and inflammation plays an important role in diabetes treatment.

Objective: To probe the Clinacanthus nutans leaf extract effect on oxidative stress and inflammatory markers and the Langerhans islet area in diabetic rat models.

Design: An experimental laboratory in the animal model.

Methods: Twenty-five diabetic rat models were randomly assigned into 5 clusters. Clusters 1, 2, and 3 were administered with C. nutans leaf extract in aqueous suspension with vehicle 1% Na-CMC at 75 mg/kg body weight (BW), 150 mg/kg BW, and 300 mg/kg BW, respectively. Cluster 4 was diabetic control rats administered with metformin at a 21 mg/rat dose. Cluster 5 was a control diabetic rat only administered with 1% Na-CMC suspension. Treatment was administered orally for 14 days. On the 15th day, the rats were sacrificed to obtain blood samples and pancreatic tissues. Serum interleukin (IL)-6, malondialdehyde (MDA), and tumor necrosis factor (TNF-α) were measured using the enzyme-linked immunosorbent assay (ELISA) method. Histopathological examination was performed by counting the Langerhans islet areas.

Results: The average IL-6, MDA, and TNF-α levels declined in the cluster receiving C. nutans extract and were significantly different from the untreated cluster (P < .05). Histopathological examination revealed a significant upsurge in the Langerhans islets area in diabetic rats receiving C. nutans extract at doses of 75 and 150 mg/kg (P < .05).

Conclusion: C. nutans leaf extract reduced the serum MDA, TNF-α, and IL-6 levels, and increased the Langerhans islets area in a diabetic rat model.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Clinacanthus nutans L提取物降低糖尿病大鼠血清肿瘤坏死因子-α、丙二醛和白细胞介素-6水平，改善朗格汉斯胰岛面积。

背景:糖尿病引起的高血糖增加了氧化应激和炎症细胞因子的产生，这在胰腺β细胞的损伤和凋亡中起重要作用。因此，减少氧化应激和炎症的药物管理在糖尿病治疗中起着重要作用。目的:探讨棘叶提取物对糖尿病大鼠氧化应激、炎症标志物及朗格汉斯岛面积的影响。设计:实验室内动物模型。方法:25只糖尿病大鼠模型随机分为5组。第1、2、3组分别以75 mg/kg体重(BW)、150 mg/kg体重(BW)、300 mg/kg体重(BW)、1% Na-CMC水溶液混悬液给药。第4组为糖尿病对照大鼠，给予二甲双胍21mg /大鼠剂量。第5组是只给予1% Na-CMC混悬液的糖尿病大鼠。口服治疗14天。第15天，处死大鼠，取血样和胰腺组织。采用酶联免疫吸附法(ELISA)检测血清白细胞介素(IL)-6、丙二醛(MDA)、肿瘤坏死因子(TNF-α)含量。通过计算朗格汉斯岛面积进行组织病理学检查。结果:枸杞子提取物组小鼠IL-6、MDA、TNF-α平均水平下降，与未治疗组差异有统计学意义(P < 0.05)。结论:枸杞子叶提取物可降低糖尿病大鼠血清MDA、TNF-α和IL-6水平，增加朗格汉斯胰岛面积。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊