Rab3a, a small GTP-binding protein, is required for the stabilization of the murine leukaemia virus Gag protein.

Q2 Biochemistry, Genetics and Molecular Biology Small GTPases Pub Date : 2022-01-01 DOI:10.1080/21541248.2021.1939631
Mai Izumida, Katsura Kakoki, Hideki Hayashi, Toshifumi Matsuyama, Yoshinao Kubo
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引用次数: 1

Abstract

We recently identified a CD63-interacting protein to understand the role of CD63 in virion production of the human immunodeficiency virus type 1, and we have found that Rab3a forms a complex with CD63. In this study, we analysed the effect of Rab3a on virion production of the murine leukaemia virus (MLV), which is another member of the retrovirus family. We found that Rab3a silencing induced lysosomal degradation of the MLV Gag protein, and recovery of the Rab3a expression restored the level of the Gag protein through a complex formation of MLV Gag and Rab3a, indicating that Rab3a is required for MLV Gag protein expression. In contrast, CD63 silencing decreased the infectivity of released virions but had no effect on virion production, indicating that CD63 facilitates the infectivity of released MLV particles. Although Rab3a induced CD63 degradation in uninfected cells, the complex of MLV Gag and Rab3a suppressed the Rab3a-mediated CD63 degradation in MLV-infected cells. Finally, we found that the MLV Gag protein interacts with Rab3a to stabilize its own protein and CD63 that facilitates the infectivity of released MLV particles. Considering the involvement of Rab3a in lysosome trafficking to the plasma membrane, it may also induce cell surface transport of the MLV Gag protein.

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Rab3a是一种小的gtp结合蛋白,是稳定小鼠白血病病毒Gag蛋白所必需的。
我们最近发现了一种CD63相互作用蛋白,以了解CD63在人类免疫缺陷病毒1型病毒粒子产生中的作用,我们发现Rab3a与CD63形成复合物。在这项研究中,我们分析了Rab3a对鼠白血病病毒(MLV)病毒粒子产生的影响,MLV是逆转录病毒家族的另一成员。我们发现Rab3a沉默诱导MLV Gag蛋白的溶酶体降解,Rab3a表达的恢复通过MLV Gag和Rab3a的复合物形成恢复Gag蛋白的水平,这表明MLV Gag蛋白的表达需要Rab3a。相比之下,CD63沉默降低了释放的病毒粒子的感染性,但对病毒粒子的产生没有影响,这表明CD63促进了释放的MLV颗粒的感染性。虽然Rab3a在未感染细胞中诱导CD63降解,但MLV Gag和Rab3a的复合物抑制了MLV感染细胞中Rab3a介导的CD63降解。最后,我们发现MLV Gag蛋白与Rab3a相互作用以稳定其自身蛋白和CD63,从而促进释放的MLV颗粒的感染性。考虑到Rab3a参与溶酶体向质膜的运输,它也可能诱导MLV Gag蛋白的细胞表面运输。
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来源期刊
Small GTPases
Small GTPases Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.10
自引率
0.00%
发文量
6
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