Xiangyu Kong, Putian An, Junping Xu, Wenzhi Liu, Feng Lin, Yulong Yang
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引用次数: 1
Abstract
Objectives: To assess A-kinase anchor protein 95 (AKAP95), B-Raf, extracellular regulated protein kinases 1/2 (ERK1/2), and Elk-1 expression in colon cancer tissue, and characterize AKAP95 associations with B-Raf, ERK1/2, Elk-1, and colon cancer clinicopathological indices.
Methods: The immunohistochemistry streptavidin-perosidase (SP) method was used to determine protein expression levels in 64 colon cancer and 32 para-carcinoma tissue specimens.
Results: (1) Positive AKAP95 expression rates in colon cancer tissue were higher when compared with para-carcinoma tissue (92.19% vs. 59.38%, P < 0.05). Similar findings were determined for B-Raf (76.56% vs. 25%, P < 0.05), ERK1/2 (90.63% vs. 31.25%, P < 0.05), and Elk-1 levels (92.19% vs. 40.63%, P < 0.05). (2) No significant associations were identified between AKAP95, B-Raf, ERK1/2, and Elk-1 protein expression and degree of differentiation, histological type, and lymph node metastasis in colon cancer samples (P > 0.05); however, in The Cancer Genome Atlas and Gene Expression Omnibus datasets, AKAP95 was closely related to immune infiltration, and highly expressed AKAP95 was negatively associated with overall survival and relapse free survival rates in colon cancer patients. (3) Correlations were observed between AKAP95 and ERK1/2, AKAP95 and Elk-1, B-Raf and ERK1/2, B-Raf and Elk-1, and ERK1/2 and Elk-1 (all P < 0.05), but no correlation was observed between AKAP95 and B-Raf (P > 0.05).
Conclusions: AKAP95 may affect immune infiltration levels in colon cancer by participating in ERK1/2-Elk-1 signal transduction.
目的:评估a激酶锚定蛋白95 (AKAP95)、B-Raf、细胞外调节蛋白激酶1/2 (ERK1/2)和Elk-1在结肠癌组织中的表达，并表征AKAP95与B-Raf、ERK1/2、Elk-1和结肠癌临床病理指标的相关性。方法:采用免疫组化streptavidin-perosidase (SP)法检测64例结肠癌和32例癌旁组织的蛋白表达水平。结果:(1)AKAP95阳性表达率在结肠癌组织中高于癌旁组织(92.19% vs. 59.38%， P < 0.05)。B-Raf (76.56% vs. 25%， P < 0.05)、ERK1/2 (90.63% vs. 31.25%， P < 0.05)和Elk-1 (92.19% vs. 40.63%， P < 0.05)的表达结果相似。(2)结肠癌组织中AKAP95、B-Raf、ERK1/2、Elk-1蛋白表达与分化程度、组织学分型、淋巴结转移无显著相关性(P > 0.05);然而，在The Cancer Genome Atlas and Gene Expression Omnibus数据集中，AKAP95与免疫浸润密切相关，且高表达的AKAP95与结肠癌患者的总生存率和无复发生存率呈负相关。(3) AKAP95与ERK1/2、AKAP95与Elk-1、B-Raf与ERK1/2、B-Raf与Elk-1、ERK1/2与Elk-1均有相关性(P < 0.05)，但与B-Raf无相关性(P > 0.05)。结论:AKAP95可能通过参与ERK1/2-Elk-1信号转导影响结肠癌免疫浸润水平。
期刊介绍：
Analytical Cellular Pathology is a peer-reviewed, Open Access journal that provides a forum for scientists, medical practitioners and pathologists working in the area of cellular pathology. The journal publishes original research articles, review articles, and clinical studies related to cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, immunopathology, and hematology.
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