The Impact of Obesity on T and NK Cells after LVAD Implantation.

IF 3.9 3区医学 Q2 ENDOCRINOLOGY & METABOLISM Obesity Facts Pub Date : 2023-01-01 Epub Date: 2023-05-11 DOI:10.1159/000530174

Eva Katharina Messer, Anna Lassia Meyer, Kristin Klaeske, Franz Sieg, Sandra Eifert, Dominik Schmiedel, Josephina Haunschild, Khalil Jawad, Diyar Saeed, Lea Hildebrandt, Michael Andrew Borger, Maja-Theresa Dieterlen

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引用次数: 1

Abstract

Introduction: Infections are a major problem after left ventricular assist device (LVAD) implantation that affects morbidity, mortality, and the quality of life. Obesity often increases the risk for infection. In the cohort of LVAD patients, it is unknown if obesity affects the immunological parameters involved in viral defense. Therefore, this study investigated whether overweight or obesity affects immunological parameters such as CD8+ T cells and natural killer (NK) cells.

Methods: Immune cell subsets of CD8+ T cells and NK cells were compared between normal-weight (BMI 18.5-24.9 kg/m2, n = 17), pre-obese (BMI 25.0-29.9 kg/m2, n = 24), and obese (BMI ≥30 kg/m2, n = 27) patients. Cell subsets and cytokine serum levels were quantified prior to LVAD implantation and at 3, 6, and 12 months after LVAD implantation.

Results: At the end of the first postoperative year, obese patients (31.8% ± 2.1%) had a lower proportion of CD8+ T cells than normal-weight patients (42.4% ± 4.1%; p = 0.04), and the percentage of CD8+ T cells was negatively correlated with BMI (p = 0.03; r = -0.329). The proportion of circulating NK cells increased after LVAD implantation patients in normal-weight (p = 0.01) and obese patients (p < 0.01). Patients with pre-obesity showed a delayed increase (p < 0.01) 12 months after LVAD implantation. Further, obese patients showed an increase in the percentage of CD57+ NK cells after 6 and 12 months (p = 0.01) of treatment, higher proportions of CD56bright NK cells (p = 0.01), and lower proportions of CD56dim/neg NK cells (p = 0.03) 3 months after LVAD implantation than normal-weight patients. The proportion of CD56bright NK cells positively correlated with BMI (p < 0.01, r = 0.403) 1 year after LVAD implantation.

Conclusions: This study documented that obesity affects CD8+ T cells and subsets of NK cells in patients with LVAD in the first year after LVAD implantation. Lower proportions of CD8+ T cells and CD56dim/neg NK cells and higher proportion of CD56bright NK cells were detected in obese but not in pre-obese and normal-weight LVAD patients during the first year after LVAD implantation. The induced immunological imbalance and phenotypic changes of T and NK cells may influence viral and bacterial immunoreactivity.

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LVAD植入术后肥胖对T细胞和NK细胞的影响。

引言：感染是左心室辅助装置（LVAD）植入后的一个主要问题，影响发病率、死亡率和生活质量。肥胖往往会增加感染的风险。在LVAD患者队列中，尚不清楚肥胖是否会影响参与病毒防御的免疫参数。因此，本研究调查了超重或肥胖是否会影响CD8+T细胞和自然杀伤细胞等免疫参数。方法：比较正常体重（BMI 18.5~24.9kg/m2，n=17）、肥胖前期（BMI 25.0~29.9kg/m2，n=24）和肥胖（BMI≥30kg/m2，n=27）患者的CD8+T细胞和NK细胞免疫细胞亚群。在LVAD植入前以及LVAD植入后3、6和12个月对细胞亚群和细胞因子血清水平进行定量。结果：术后第一年末，肥胖患者（31.8%±2.1%）的CD8+T细胞比例低于正常体重患者（42.4%±4.1%；p=0.04），CD8+T细胞百分比与BMI呈负相关（p=0.03；r=-0.329）。LVAD植入后，正常体重患者和肥胖患者的循环NK细胞比例增加（p=0.01）。肥胖前期患者在LVAD植入12个月后出现延迟性增加（p<0.01）。此外，与正常体重患者相比，肥胖患者在LVAD植入后6个月和12个月的治疗后CD57+NK细胞的百分比增加（p＝0.01），CD56bright NK细胞的比例增加（p＜0.01），并且CD56dim/neg NK细胞的比例降低（p＝0.03）。LVAD植入1年后，CD56bright NK细胞的比例与BMI呈正相关（p＜0.01，r＝0.403）。结论：本研究表明，在LVAD植入后的第一年，肥胖会影响LVAD患者的CD8+T细胞和NK细胞亚群。在LVAD植入后的第一年，在肥胖患者中检测到较低比例的CD8+T细胞和CD56dim/neg NK细胞，以及较高比例的CD56bright NK细胞，但在肥胖前期和正常体重的LVAD患者中没有检测到。诱导的免疫失衡和T细胞和NK细胞的表型变化可能影响病毒和细菌的免疫反应性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Obesity Facts 医学-内分泌学与代谢

CiteScore

6.80

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Obesity Facts'' publishes articles covering all aspects of obesity, in particular epidemiology, etiology and pathogenesis, treatment, and the prevention of adiposity. As obesity is related to many disease processes, the journal is also dedicated to all topics pertaining to comorbidity and covers psychological and sociocultural aspects as well as influences of nutrition and exercise on body weight. The editors carefully select papers to present only the most recent findings in clinical practice and research. All professionals concerned with obesity issues will find this journal a most valuable update to keep them abreast of the latest scientific developments.