Colletotrichum orbiculare strains distributed in Japan: race identification and evaluation of virulence to cucurbits.

IF 2 4区 农林科学 Q2 AGRONOMY Breeding Science Pub Date : 2022-09-01 DOI:10.1270/jsbbs.22011
Hiroki Matsuo, Yasuhiro Ishiga, Yasuyuki Kubo, Yosuke Yoshioka
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引用次数: 1

Abstract

The pathogen Colletotrichum orbiculare is causal fungus of cucurbit anthracnose. Multiple races have been identified in the United States, suggesting that it is necessary to cultivate suitable resistant cultivars and breed new cultivars with the most suitable resistance gene. This study examined the pathogenicity and virulence of 20 strains in Japan to clarify the existence of races and virulence differences. Based on the symptoms on inoculated cotyledons and true leaves of watermelon, we could evaluate the compatibility of each strain to each host cultivar. Our analysis based on the reaction to the host cultivar harboring the resistance gene Ar-1 (Cla001017) revealed the existence of three races in Japan. An alarming result was that a race that overcame Ar-1, which is a target gene in current watermelon breeding in Japan, is present in Japan. The cucumber and melon host cultivars showed diverse symptoms, whereas a squash cultivar was resistant to all strains. Three strains caused severe damage even to the most resistant cucumber cultivar 'Ban Kyuri' and resistant cultivars harboring Cssgr, a well-known gene conferring loss-of-susceptibility resistance. Screening genetic resources for novel resistance genes using strains with high virulence is of vital importance for watermelon, cucumber, and melon production.

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分布于日本的圆形炭疽病菌:小种鉴定及对葫芦的毒力评价。
葫芦丝炭疽病菌是引起葫芦丝炭疽病的病原菌。在美国已经发现了多个品种,这表明有必要培育合适的抗性品种,并培育具有最合适抗性基因的新品种。本研究检测了日本20株菌株的致病性和毒力,以澄清种族的存在和毒力差异。根据西瓜接种子叶和真叶的症状,可以评价各菌株与各寄主品种的亲和性。通过对携带Ar-1抗性基因的寄主品种(cl001017)的反应分析,发现在日本存在3个小种。一个令人担忧的结果是,日本出现了一种克服Ar-1基因的品种,Ar-1基因是目前日本西瓜育种的目标基因。黄瓜和甜瓜宿主品种表现出不同的症状,而南瓜品种对所有菌株都具有抗性。三种菌株甚至对最具抗性的黄瓜品种“Ban Kyuri”和含有Cssgr的抗性品种造成了严重损害,Cssgr是一种众所周知的具有丧失敏感性抗性的基因。利用高毒力菌株筛选新抗性基因的遗传资源对西瓜、黄瓜和甜瓜生产具有重要意义。
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来源期刊
Breeding Science
Breeding Science 农林科学-农艺学
CiteScore
4.90
自引率
4.20%
发文量
37
审稿时长
1.5 months
期刊介绍: Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews related to breeding. Research Papers are standard original articles. Notes report new cultivars, breeding lines, germplasms, genetic stocks, mapping populations, database, software, and techniques significant and useful for breeding. Reviews summarize recent and historical events related breeding. Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.
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