Basic amino acid-mediated cationic amphiphilic surfaces for antimicrobial pH monitoring sensor with wound healing effects.

IF 11.3 1区 医学 Q1 Medicine Biomaterials Research Pub Date : 2023-02-17 DOI:10.1186/s40824-023-00355-0
Dong Uk Lee, Se-Chang Kim, Dong Yun Choi, Won-Kyo Jung, Myung Jun Moon
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Abstract

Background: The wound healing process is a complex cascade of physiological events, which are vulnerable to both our body status and external factors and whose impairment could lead to chronic wounds or wound healing impediments. Conventional wound healing materials are widely used in clinical management, however, they do not usually prevent wounds from being infected by bacteria or viruses. Therefore, simultaneous wound status monitoring and prevention of microbial infection are required to promote healing in clinical wound management.

Methods: Basic amino acid-modified surfaces were fabricated in a water-based process via a peptide coupling reaction. Specimens were analyzed and characterized by X-ray photoelectron spectroscopy, Kelvin probe force microscopy, atomic force microscopy, contact angle, and molecular electrostatic potential via Gaussian 09. Antimicrobial and biofilm inhibition tests were conducted on Escherichia coli and Staphylococcus epidermidis. Biocompatibility was determined through cytotoxicity tests on human epithelial keratinocytes and human dermal fibroblasts. Wound healing efficacy was confirmed by mouse wound healing and cell staining tests. Workability of the pH sensor on basic amino acid-modified surfaces was evaluated on normal human skin and Staphylococcus epidermidis suspension, and in vivo conditions.

Results: Basic amino acids (lysine and arginine) have pH-dependent zwitterionic functional groups. The basic amino acid-modified surfaces had antifouling and antimicrobial properties similar to those of cationic antimicrobial peptides because zwitterionic functional groups have intrinsic cationic amphiphilic characteristics. Compared with untreated polyimide and modified anionic acid (leucine), basic amino acid-modified polyimide surfaces displayed excellent bactericidal, antifouling (reduction ~ 99.6%) and biofilm inhibition performance. The basic amino acid-modified polyimide surfaces also exhibited wound healing efficacy and excellent biocompatibility, confirmed by cytotoxicity and ICR mouse wound healing tests. The basic amino acid-modified surface-based pH monitoring sensor was workable (sensitivity 20 mV pH-1) under various pH and bacterial contamination conditions.

Conclusion: Here, we developed a biocompatible and pH-monitorable wound healing dressing with antimicrobial activity via basic amino acid-mediated surface modification, creating cationic amphiphilic surfaces. Basic amino acid-modified polyimide is promising for monitoring wounds, protecting them from microbial infection, and promoting their healing. Our findings are expected to contribute to wound management and could be expanded to various wearable healthcare devices for clinical, biomedical, and healthcare applications.

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碱性氨基酸介导的阳离子两亲性表面用于具有伤口愈合作用的抗菌pH监测传感器。
背景:创面愈合过程是一个复杂的级联生理事件,易受机体状态和外界因素的影响,其损伤可导致慢性创面或创面愈合障碍。传统的伤口愈合材料在临床治疗中被广泛使用,然而,它们通常不能防止伤口被细菌或病毒感染。因此,在临床创面管理中,需要同时监测创面状态和预防微生物感染,以促进创面愈合。方法:通过多肽偶联反应,在水基法制备碱性氨基酸修饰表面。采用x射线光电子能谱、开尔文探针力显微镜、原子力显微镜、接触角和分子静电势等方法对样品进行了分析和表征。对大肠杆菌和表皮葡萄球菌进行了抑菌试验和生物膜抑制试验。通过人上皮角质形成细胞和人真皮成纤维细胞的细胞毒性试验确定生物相容性。小鼠创面愈合及细胞染色试验证实创面愈合效果。在正常人体皮肤和表皮葡萄球菌悬浮液以及体内条件下,评估了pH传感器在碱性氨基酸修饰表面的可加工性。结果:碱性氨基酸(赖氨酸和精氨酸)具有ph依赖的两性离子官能团。由于两性离子官能团具有固有的阳离子两亲性,碱性氨基酸修饰的表面具有与阳离子抗菌肽相似的防污和抗菌性能。与未处理的聚酰亚胺和改性阴离子酸(亮氨酸)相比,碱性氨基酸改性聚酰亚胺表面表现出优异的杀菌、防污(还原~ 99.6%)和生物膜抑制性能。细胞毒性和ICR小鼠伤口愈合实验证实,碱性氨基酸修饰的聚酰亚胺表面也表现出良好的伤口愈合效果和生物相容性。碱性氨基酸修饰的表面pH监测传感器在各种pH和细菌污染条件下均可工作,灵敏度为20 mV pH-1。结论:通过碱性氨基酸介导的表面修饰,我们开发了一种具有生物相容性和ph可监测的抗菌活性伤口愈合敷料,形成了阳离子两亲性表面。碱性氨基酸修饰聚酰亚胺有望监测伤口,保护他们免受微生物感染,并促进其愈合。我们的研究结果有望为伤口管理做出贡献,并可扩展到临床、生物医学和医疗保健应用的各种可穿戴医疗设备。
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来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
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