{"title":"Discovery of seven hox genes in zebrafish thrombopoiesis","authors":"Hemalatha Sundaramoorthi, Weam Fallatah, Jabila Mary, Pudur Jagadeeswaran","doi":"10.1016/j.bcmd.2023.102796","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>Thrombopoiesis is the production of platelets from </span>megakaryocytes<span> in the bone marrow of mammals. In fish, thrombopoiesis involves the formation of thrombocytes without megakaryocyte-like precursors but derived from erythrocyte thrombocyte bi-functional precursor cells. One unique feature of thrombocyte differentiation involves the maturation of young thrombocytes in circulation. In this study, we investigated the role of </span></span><em>hox</em> genes in zebrafish thrombopoiesis to model platelet production. We selected <em>hoxa10b</em>, <em>hoxb2a</em>, <em>hoxc5a</em>, <em>hoxd3a,</em> and <em>hoxc11b</em><span> from thrombocyte RNA expression data, and checked whether they are expressed in young or mature thrombocytes. We found </span><em>hoxa10b</em>, <em>hoxb2a</em>, <em>hoxc5a</em>, and <em>hoxd3a</em> were expressed in both young and mature thrombocytes and <em>hoxc11b</em> was expressed in only young thrombocytes. We then performed knockdowns of these 5 <em>hox</em> genes and found <em>hoxc11b</em><span><span> knockdown resulted in thrombocytosis and the rest showed </span>thrombocytopenia. To identify </span><em>hox</em> genes that could have been missed by the above datasets, we performed knockdowns 47 <em>hox</em> genes in the zebrafish genome and found <em>hoxa9a</em>, and <em>hoxb1a</em> knockdowns resulted in thrombocytopenia and they were expressed in both young and mature thrombocytes. In conclusion, our comprehensive knockdown study identified Hoxa10b, Hoxb2a, Hoxc5a, Hoxd3a, Hoxa9a, and Hoxb1a, as positive regulators and Hoxc11b<em>,</em> as a negative regulator for thrombocyte development.</p></div>","PeriodicalId":8972,"journal":{"name":"Blood Cells Molecules and Diseases","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Blood Cells Molecules and Diseases","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1079979623000736","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Thrombopoiesis is the production of platelets from megakaryocytes in the bone marrow of mammals. In fish, thrombopoiesis involves the formation of thrombocytes without megakaryocyte-like precursors but derived from erythrocyte thrombocyte bi-functional precursor cells. One unique feature of thrombocyte differentiation involves the maturation of young thrombocytes in circulation. In this study, we investigated the role of hox genes in zebrafish thrombopoiesis to model platelet production. We selected hoxa10b, hoxb2a, hoxc5a, hoxd3a, and hoxc11b from thrombocyte RNA expression data, and checked whether they are expressed in young or mature thrombocytes. We found hoxa10b, hoxb2a, hoxc5a, and hoxd3a were expressed in both young and mature thrombocytes and hoxc11b was expressed in only young thrombocytes. We then performed knockdowns of these 5 hox genes and found hoxc11b knockdown resulted in thrombocytosis and the rest showed thrombocytopenia. To identify hox genes that could have been missed by the above datasets, we performed knockdowns 47 hox genes in the zebrafish genome and found hoxa9a, and hoxb1a knockdowns resulted in thrombocytopenia and they were expressed in both young and mature thrombocytes. In conclusion, our comprehensive knockdown study identified Hoxa10b, Hoxb2a, Hoxc5a, Hoxd3a, Hoxa9a, and Hoxb1a, as positive regulators and Hoxc11b, as a negative regulator for thrombocyte development.
期刊介绍:
Blood Cells, Molecules & Diseases emphasizes not only blood cells, but also covers the molecular basis of hematologic disease and studies of the diseases themselves. This is an invaluable resource to all those interested in the study of hematology, cell biology, immunology, and human genetics.