Immune-based prognostic biomarkers associated with metastasis of osteosarcoma.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.4149/gpb_2022050

Teng Ma, Changliang Peng, Dongjin Wu, Song Yang, Li Ji, Zhang Cheng, Chunzheng Gao

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Abstract

This study aimed to identify immune-based prognostic biomarkers associated with metastasis of osteosarcoma. Based on the GEO and TCGA databases, 437 differentially expressed genes were screened between primary and metastatic osteosarcoma. Weighted gene co-expression network analysis (WGCNA) revealed 496 genes in turquoise module which had the highest correlation with osteosarcoma metastasis. Within these two group genes, 122 common genes involved in osteosarcoma metastasis were identified. These genes were enriched in chemokine activity, chemokine receptor binding, TNF signaling pathway, etc. Survival analysis revealed 8 prognostic genes (ANK3, EGR1, FBP1, FOS, KIFC3, MAOB, ISLR and MFAP4) from the 122 genes. RT-qPCR showed that all of these eight genes were differentially expressed between 143B and MNNG/HOS Cl cells. Various infiltrating immune cells showed significant differences between primary and metastatic osteosarcoma. Expression of all the 8 prognostic genes was correlated with infiltration abundance of multiple immune cells, such as follicular helper T cells, activated dendritic cells. In addition, 10 microRNAs and 7 transcription factors that targeted these prognostic genes were predicted. In conclusion, 8 immune-based prognostic genes associated with osteosarcoma metastasis were identified.

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与骨肉瘤转移相关的免疫预后生物标志物。

本研究旨在确定与骨肉瘤转移相关的基于免疫的预后生物标志物。基于GEO和TCGA数据库，筛选了原发性和转移性骨肉瘤之间的437个差异表达基因。加权基因共表达网络分析(WGCNA)显示，绿松石模块中496个基因与骨肉瘤转移相关性最高。在这两组基因中，鉴定出122个与骨肉瘤转移相关的常见基因。这些基因在趋化因子活性、趋化因子受体结合、TNF信号通路等方面富集。生存分析显示122个基因中有8个预后基因(ANK3、EGR1、FBP1、FOS、KIFC3、MAOB、ISLR和MFAP4)。RT-qPCR结果显示，这8个基因在143B细胞和MNNG/HOS Cl细胞中均有差异表达。各种浸润性免疫细胞在原发性骨肉瘤和转移性骨肉瘤中表现出显著差异。8种预后基因的表达均与滤泡辅助性T细胞、活化的树突状细胞等多种免疫细胞浸润丰度相关。此外，我们还预测了针对这些预后基因的10个microrna和7个转录因子。总之，我们确定了8个与骨肉瘤转移相关的基于免疫的预后基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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