The preventive effect of Mori Ramulus on oxidative stress-induced cellular damage in skeletal L6 myoblasts through Nrf2-mediated activation of HO-1.

IF 1.6 4区医学 Q4 TOXICOLOGY Toxicological Research Pub Date : 2022-07-22 eCollection Date: 2023-01-01 DOI:10.1007/s43188-022-00141-5

Cheol Park, Hyesook Lee, Sung Ok Kim, Eun-Woo Lee, Hyun-Tai Lee, Hyun Ju Kwon, Byung Woo Kim, Gi-Young Kim, Mi Ryeo Kim, Yung Hyun Choi

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引用次数: 3

Abstract

The aim of the present study is to investigate the preventive effect of water extract of Mori Ramulus (MRWE) on oxidative stress-mediated cellular damages in rat skeletal L6 myoblasts. Our results demonstrated that MRWE pretreatment markedly improved cell survival and suppressed cell cycle arrest at the G2/M phase and apoptosis in hydrogen peroxide (H₂O₂)-treated L6 cells. H₂O₂-triggered DNA damage was also notably reduced by MRWE, which since it was correlated with protection of reactive oxygen species (ROS) production. Additionally, H₂O₂ stimulated cytosolic release of cytochrome c and up-regulation of Bax/Bcl-2 ratio, whereas MRWE suppressed these changes following by H₂O₂. Moreover, MRWE inhibited the cleavage of poly(ADP-ribose) polymerase as well as the activity of caspase-3 by H₂O₂. Furthermore, MRWE enhanced H₂O₂-mediated expression of nuclear factor erythroid 2-associated factor 2 (Nrf2) and its representative downstream enzyme, heme oxygenase-1 (HO-1). However, the protective effects of MRWE on H₂O₂-induced ROS production, cell cycle arrest and apoptosis were significantly attenuated by HO-1 inhibitor. In conclusion, our present results suggests that MRWE could protect L6 myoblasts from H₂O₂-induced cellular injury by inhibiting ROS generation along with Nrf2-mediated activation of HO-1, indicating this finding may expand the scope of application of Mori Ramulus in medicine.

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森罗木麻通过 Nrf2 介导的 HO-1 激活，对氧化应激诱导的骨骼 L6 肌母细胞细胞损伤具有预防作用。

本研究旨在探讨森獐牙菜水提取物（MRWE）对氧化应激介导的大鼠骨骼L6肌细胞损伤的预防作用。结果表明，在过氧化氢（H2O2）处理的 L6 细胞中，MRWE 预处理显著提高了细胞存活率，抑制了 G2/M 期细胞周期停滞和细胞凋亡。MRWE还显著减少了H2O2引发的DNA损伤，这与保护活性氧（ROS）的产生有关。此外，H2O2刺激细胞膜释放细胞色素c，并上调Bax/Bcl-2比率，而MRWE抑制了H2O2引起的这些变化。此外，MRWE 还能抑制 H2O2 对聚（ADP-核糖）聚合酶的裂解以及 caspase-3 的活性。此外，MRWE 还增强了 H2O2 介导的核因子红细胞 2 相关因子 2（Nrf2）及其代表性下游酶血红素加氧酶 1（HO-1）的表达。然而，HO-1抑制剂显著削弱了MRWE对H2O2诱导的ROS产生、细胞周期停滞和细胞凋亡的保护作用。总之，我们目前的研究结果表明，MRWE 可以通过抑制 ROS 生成和 Nrf2 介导的 HO-1 激活来保护 L6 肌母细胞免受 H2O2 诱导的细胞损伤，这一发现可能会扩大森罗木素在医学中的应用范围。

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来源期刊

Toxicological Research TOXICOLOGY-

CiteScore

4.20

自引率

4.30%

发文量

期刊介绍： Toxicological Research is the official journal of the Korean Society of Toxicology. The journal covers all areas of Toxicological Research of chemicals, drugs and environmental agents affecting human and animals, which in turn impact public health. The journal’s mission is to disseminate scientific and technical information on diverse areas of toxicological research. Contributions by toxicologists, molecular biologists, geneticists, biochemists, pharmacologists, clinical researchers and epidemiologists with a global view on public health through toxicological research are welcome. Emphasis will be given to articles providing an understanding of the toxicological mechanisms affecting animal, human and public health. In the case of research articles using natural extracts, detailed information with respect to the origin, extraction method, chemical profiles, and characterization of standard compounds to ensure the reproducible pharmacological activity should be provided.