A DEK domain-containing protein GhDEK2D mediated Gossypium hirsutum enhanced resistance to Verticillium dahliae.

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Plant Signaling & Behavior Pub Date : 2022-12-31 DOI:10.1080/15592324.2021.2024738

Jinglong Zhou, Lihong Zhao, Yajie Wu, Xiaojian Zhang, Sheng Cheng, Feng Wei, Yalin Zhang, Heqin Zhu, Yi Zhou, Zili Feng, Hongjie Feng

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引用次数: 4

Abstract

DEK is associated with DNA replication and break repair, mRNA splicing, and transcriptional regulation, which had been studied in humans and mammals. The function of DEK in plants was poorly understood. In this study, GhDEK2D was identified in Gossypium hirsutum by genome-wide and post-translational modifications. GhDEK2D had been phosphorylated, acetylated and ubiquitylated under Verticillium dahliae (Vd) challenge. The GhDEK2D-silenced cotton decreased resistance against Vd. In GhDEK2D-silenced cotton plants, the reactive oxygen species was activated, the callose, xylogen, hypersensitive reaction (HR) and expression levels of defense-related genes were reduced. Homozygous overexpressing-GhDEK2D transgenic Arabidopsis lines were more resistant to Verticillium wilt (Vw). We propose that GhDEK2D was a potential molecular target for improving resistance to Vw in cotton.

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含有DEK结构域的蛋白GhDEK2D介导的棉花增强了对大丽花黄萎病的抗性。

DEK与DNA复制和断裂修复、mRNA剪接和转录调控有关，已在人类和哺乳动物中进行了研究。DEK在植物中的作用尚不清楚。在这项研究中，通过全基因组和翻译后修饰，在绵棉中鉴定出了GhDEK2D。GhDEK2D在大丽花黄萎病菌(Verticillium dahliae, Vd)侵染下发生磷酸化、乙酰化和泛素化。ghdek2d沉默棉降低了对Vd的抗性。在ghdek2d沉默的棉花植株中，活性氧被激活，胼胝质、木质、超敏反应(hypersensitive reaction, HR)和防御相关基因的表达水平降低。纯合子过表达ghdek2d转基因拟南芥株系对黄萎病(Verticillium wilt, Vw)的抗性更强。我们认为GhDEK2D是提高棉花对Vw抗性的潜在分子靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Signaling & Behavior Agricultural and Biological Sciences-Plant Science

CiteScore

6.00

自引率

3.40%

发文量

111

期刊介绍： Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.