Toxicological signature for thyroid endocrine disruption of dichlorooctylisothiazolinone in zebrafish larvae.

IF 2.4 4区环境科学与生态学 Q2 ECOLOGY Ecotoxicology Pub Date : 2023-01-01 DOI:10.1007/s10646-022-02614-7

Sujin Lee, Kyunghee Ji

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引用次数: 3

Abstract

Dichlorooctylisothiazolinone (DCOIT), which is one of the isothiazolinone preservatives, is applied to water-based adhesives in food packaging. This study investigated the effects of DCOIT on the embryonic growth and thyroid endocrine system using zebrafish. Organism-level (hatchability, survival, and growth), hormone-level (triiodothyronine (T3) and thyroxine (T4)), gene-level (genes associated with the hypothalamus-pituitary-thyroid axis), and microRNA-level (microRNAs related to thyroid endocrine disruption) endpoints were measured. Significant rise in embryonic coagulation and delayed hatching (≥0.3 μg/L), and decreased larval length (30 μg/L) were observed in fish exposed to DCOIT. Lower contents of T3 and T4 were observed after exposure to DCOIT, which was accompanied by the upregulation of genes associated with the thyrotropin releasing hormone and thyroid stimulating hormone and the downregulation of genes associated with the thyroid hormone receptors and deiodination. Strong influence of DCOIT on dre-miR-193b and -499 may be a critical mechanism to inhibit transcription of trαa and trβ, which in turn may affect thyroid hormones and development of the organism. Our findings suggest that hypothyroidism induced by the exposure to DCOIT is potentially associated with genetic and microRNA-level changes, which eventually affects development.

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二氯辛基异噻唑啉酮对斑马鱼幼虫甲状腺内分泌干扰的毒理学特征。

二氯辛基基异噻唑啉酮(DCOIT)是一种异噻唑啉酮类防腐剂，主要应用于食品包装的水性粘合剂中。本研究以斑马鱼为实验对象，研究了DCOIT对胚胎生长和甲状腺内分泌系统的影响。测量生物水平(孵化率、存活率和生长)、激素水平(三碘甲状腺原氨酸(T3)和甲状腺素(T4))、基因水平(与下丘脑-垂体-甲状腺轴相关的基因)和微rna水平(与甲状腺内分泌干扰相关的微rna)终点。DCOIT显著提高了胚胎凝固和延迟孵化率(≥0.3 μg/L)，降低了幼虫长度(30 μg/L)。DCOIT暴露后T3、T4含量降低，促甲状腺激素释放激素和促甲状腺激素相关基因上调，甲状腺激素受体和去碘相关基因下调。DCOIT对re- mir -193b和-499的强烈影响可能是抑制trαa和trβ转录的关键机制，而trαa和trβ转录反过来可能影响甲状腺激素和生物体的发育。我们的研究结果表明，暴露于DCOIT引起的甲状腺功能减退可能与遗传和microrna水平的变化有关，最终影响发育。

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来源期刊

Ecotoxicology 环境科学-毒理学

CiteScore

5.30

自引率

3.70%

发文量

107

审稿时长

4.7 months

期刊介绍： Ecotoxicology is an international journal devoted to the publication of fundamental research on the effects of toxic chemicals on populations, communities and terrestrial, freshwater and marine ecosystems. It aims to elucidate mechanisms and processes whereby chemicals exert their effects on ecosystems and the impact caused at the population or community level. The journal is not biased with respect to taxon or biome, and papers that indicate possible new approaches to regulation and control of toxic chemicals and those aiding in formulating ways of conserving threatened species are particularly welcome. Studies on individuals should demonstrate linkage to population effects in clear and quantitative ways. Laboratory studies must show a clear linkage to specific field situations. The journal includes not only original research papers but technical notes and review articles, both invited and submitted. A strong, broadly based editorial board ensures as wide an international coverage as possible.