Santi Chismirina, Suzanna Sungkar, Muhammad Adlim, Darmawi Darmawi
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引用次数: 0
Abstract
Background: Dental caries is an oral disease that is widely suffered by the population of Aceh caused by Streptococcus mutans. S. mutans serotypes c and d are widely isolated in the human oral cavity. This research was focused on detecting the presence and variability of S. mutans in supragingival dental plaque of caries teenager and young adults' patients.
Methods: Subjects involved in this study were patients who treated at the Rumah Sakit Gigi dan Mulut of Dentistry Faculty of Universitas Syiah Kuala. The approach used in this research was molecular microbiology technique. To determine the presence of S. mutans, supragingival plaque from caries patients was cultivated in TYS20B. The culture findings were utilized to detect the presence of bacteria using PCR. The primers utilized in the PCR were S. mutans specific primers, GTFB (517 bp) for S. mutans serotype c and GTFI (712 bp) for S. mutans serotype d.
Results: Culture results on TYS20B media showed the growth of S. mutans colonies isolated from the supragingival plaque of research subjects. PCR results also revealed the presence of S. mutans in the supragingival plaques of caries patients, with the variability of S. mutans discovered to be a serotype c and a serotype d.
Conclusion: Based on the findings of this study, it can be concluded that S. mutans can be found in the supragingival plaques of caries patients with the serotypes c and d variability.
背景:龋齿是由变形链球菌引起的亚齐省人口普遍患病的一种口腔疾病。变异链球菌血清型c和d在人类口腔中广泛分离。本研究的重点是检测青少年和年轻人龋齿患者龈上牙菌斑中变异链球菌的存在和变异性。方法:参与本研究的受试者是在吉隆坡大学牙科学院Rumah Sakit Gigi dan Mulut接受治疗的患者。这项研究采用的方法是分子微生物学技术。为了确定变异链球菌的存在,在TYS20B中培养来自龋齿患者的龈上菌斑。培养结果用于使用PCR检测细菌的存在。PCR中使用的引物是变异链球菌特异性引物,GTFB(517bp)用于c型变异链球菌,GTFI(712bp)用于d型变异链球菌。PCR结果还显示,龋齿患者的龈上斑块中存在变异链球菌,变异链球菌分为c型和d型。
期刊介绍:
The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.