Aberrant Methylation of the SOD1 GENE, its Expression and Enzyme Activity in the Placenta of Patients with Preeclampsia.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-04-01 DOI:10.52547/rbmb.12.1.112
Saba Zakeri, Zohreh Rahimi, Nazanin Jalilian, Asad Vaisi-Raygani, Ali Rezvani, Zahra Dastafkan
{"title":"Aberrant Methylation of the <i>SOD1</i> GENE, its Expression and Enzyme Activity in the Placenta of Patients with Preeclampsia.","authors":"Saba Zakeri,&nbsp;Zohreh Rahimi,&nbsp;Nazanin Jalilian,&nbsp;Asad Vaisi-Raygani,&nbsp;Ali Rezvani,&nbsp;Zahra Dastafkan","doi":"10.52547/rbmb.12.1.112","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Oxidative stress is involved in the pathogenesis of preeclampsia (PE). Dysregulation of <i>SOD1</i> may be involved in the pathogenesis of PE. We examined and compared the methylation level of the promoter region (PMR) of the <i>SOD1</i>, gene expression, and enzyme activity of superoxide dismutase (SOD) in both placenta and maternal blood in PE women.</p><p><strong>Methods: </strong>A total of 140 blood samples and 40 placental tissue samples from PE and healthy pregnant controls were studied. The PMR of the <i>SOD1</i> (Methylight PCR method), the expression (Real-time PCR), and its enzyme activity were investigated and compared in two groups.</p><p><strong>Results: </strong>The PMR of the <i>SOD1</i> gene in the placental tissue of the patients was significantly increased compared to the control group (P= 0.008); this result was accompanied by a decrease in the expression of the gene and a decrease in the activity of the SOD enzyme. Meanwhile, the PMR of the <i>SOD1</i> gene did not significantly change in the blood samples of the patients (P= 0.95), while a significant decrease in the expression of SOD1 (without a significant change in the SOD activity) was observed.</p><p><strong>Conclusion: </strong>The results showed significant changes in the PMR of the <i>SOD1</i> gene and gene expression in placenta tissue. The results highlight the role of the placenta in complications during pregnancy and also revealed epigenetics as an important regulatory pathway in the pathogenesis of preeclampsia.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 1","pages":"112-119"},"PeriodicalIF":1.6000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505468/pdf/rbmb-12-112.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reports of Biochemistry and Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.52547/rbmb.12.1.112","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Oxidative stress is involved in the pathogenesis of preeclampsia (PE). Dysregulation of SOD1 may be involved in the pathogenesis of PE. We examined and compared the methylation level of the promoter region (PMR) of the SOD1, gene expression, and enzyme activity of superoxide dismutase (SOD) in both placenta and maternal blood in PE women.

Methods: A total of 140 blood samples and 40 placental tissue samples from PE and healthy pregnant controls were studied. The PMR of the SOD1 (Methylight PCR method), the expression (Real-time PCR), and its enzyme activity were investigated and compared in two groups.

Results: The PMR of the SOD1 gene in the placental tissue of the patients was significantly increased compared to the control group (P= 0.008); this result was accompanied by a decrease in the expression of the gene and a decrease in the activity of the SOD enzyme. Meanwhile, the PMR of the SOD1 gene did not significantly change in the blood samples of the patients (P= 0.95), while a significant decrease in the expression of SOD1 (without a significant change in the SOD activity) was observed.

Conclusion: The results showed significant changes in the PMR of the SOD1 gene and gene expression in placenta tissue. The results highlight the role of the placenta in complications during pregnancy and also revealed epigenetics as an important regulatory pathway in the pathogenesis of preeclampsia.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
先兆子痫患者胎盘SOD1基因异常甲基化及其表达和酶活性。
背景:氧化应激参与了子痫前期(PE)的发病机制。SOD1的失调可能参与PE的发病机制。我们检测并比较了PE妇女胎盘和母体血液中SOD1启动子区(PMR)的甲基化水平、基因表达和超氧化物歧化酶(SOD)的酶活性。方法:对来自PE和健康孕妇对照的140份血液样本和40份胎盘组织样本进行研究。研究并比较两组SOD1的PMR(Methylight PCR法)、表达(Real-time PCR)及其酶活性。结果:与对照组相比,患者胎盘组织中SOD1基因的PMR显著升高(P=0.008);该结果伴随着基因表达的降低和SOD酶活性的降低。同时,SOD1基因的PMR在患者的血液样本中没有显著变化(P=0.95),而SOD1的表达显著降低(SOD活性没有显著变化)。结论:SOD1基因的PMR和基因在胎盘组织中的表达发生了显著变化。研究结果强调了胎盘在妊娠并发症中的作用,并揭示了表观遗传学在先兆子痫发病机制中的重要调控途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
期刊最新文献
Association of ABCB1(Rs10276036, C/T) Gene, IL-18, and TNFα as Risk Factors for Nephrotic Syndrome Incidence. Association of Body Mass Index with Matrix Metalloproteinase-9, Tissue Inhibitor of Metalloproteinase-1, and Interleukin-6 Based on Blood Pressure. Association of lncRNA ANRIL rs10757278 A>G Variant, Tumor Size, Grading, Tumor Site, and Tumor Stage in Oral Squamous Cell Carcinoma Patients. Association of Serum Anti-Mullerian Hormone and Free Testosterone with Different Phenotypes of Polycystic Ovary Syndrome. Catechin Hydrate Improves Hypertrophic Scar in Rabbit Ear Model via Reduction of Collagen Synthesis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1