RNA m6A modification: Mapping methods, roles, and mechanisms in acute myeloid leukemia.

Abstract

N⁶-Methyladenosine (m⁶A) is the most abundant modification in eukaryotic mRNA, and plays important biological functions via regulating RNA fate determination. Recent studies have shown that m⁶A modification plays a key role in hematologic malignancies, including acute myeloid leukemia. The current growth of epitranscriptomic research mainly benefits from technological progress in detecting RNA m⁶A modification in a transcriptome-wide manner. In this review, we first briefly summarize the latest advances in RNA m⁶A biology by focusing on writers, readers, and erasers of m⁶A modification, and describe the development of high-throughput methods for RNA m⁶A mapping. We further discuss the important roles of m⁶A modifiers in acute myeloid leukemia, and highlight the identification of potential inhibitors for AML treatment by targeting of m⁶A modifiers. Overall, this review provides a comprehensive summary of RNA m⁶A biology in acute myeloid leukemia.