{"title":"Purification of Functional Gene Transfer Agents Using Two-Step Preparative Monolithic Chromatography.","authors":"Evan Langille, Christina S Bottaro, Andrew S Lang","doi":"10.1089/phage.2022.0035","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Gene transfer agents (GTAs) are phage-like particles that transfer cellular genomic DNA between cells. A hurdle faced in studying GTA function and interactions with cells is the difficulty in obtaining pure and functional GTAs from cultures.</p><p><strong>Materials and methods: </strong>We used a novel two-step method for purification of GTAs from <i>R. capsulatus</i> by monolithic chromatography.</p><p><strong>Results: </strong>Our efficient and simple process had advantages compared to previous approaches. The purified GTAs retained gene transfer activity and the packaged DNA could be used for further studies.</p><p><strong>Conclusions: </strong>This method is applicable to GTAs produced by other species and small phages, and could be useful for therapeutic applications.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"3 4","pages":"194-203"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917305/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PHAGE (New Rochelle, N.Y.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/phage.2022.0035","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/12/19 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Gene transfer agents (GTAs) are phage-like particles that transfer cellular genomic DNA between cells. A hurdle faced in studying GTA function and interactions with cells is the difficulty in obtaining pure and functional GTAs from cultures.
Materials and methods: We used a novel two-step method for purification of GTAs from R. capsulatus by monolithic chromatography.
Results: Our efficient and simple process had advantages compared to previous approaches. The purified GTAs retained gene transfer activity and the packaged DNA could be used for further studies.
Conclusions: This method is applicable to GTAs produced by other species and small phages, and could be useful for therapeutic applications.
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