[miR-141-3p enhances the proliferation, invasion and migration of CNE-2 human nasopharyngeal carcinoma cells by promoting vimentin expression].

Abstract

Objective To investigate whether miR-141-3p promote the migration of CNE-2 human nasopharyngeal carcinoma (NPC) cells by regulating vimentin. Methods Real-time quantitative PCR was used to detect the expression of miR-141-3p in NPC tissues and adjacent tissues and the expression level of vimentin was detected by immunohistochemical staining and Western blot analysis. Real-time quantitative PCR and Western blot were used to screen 5-8F, CNE-2, HNE1 human NPC cell lines with the highest relative expression of miR-141-3p. Real-time quantitative PCR and Western blot analysis were used together to verify the relationship between miR-141-3p and vimentin expression. Small interfering RNA (si-miR-141-3p) was used to down-regulate miR-141-3p of CNE-2 cells. MTT assay tested the proliferating inhibition rate of CNE-2 cells. Transwell^TM chamber assay was performed to detect cell invasion and migration and Western blot analysis to detect the expression of vimentin. Results Compared with the paracancerous tissues, the expression of miR-141-3p and vimentin in NPC tissues increased significantly. Compared with NP69 cells, the expressions of miR-141-3p and vimentin increased significantly in CNE-2 cells. The down-regulation of miR-141-3p in CNE-2 cells has induced significant decrease of cell invasion and migration capabilities, cell proliferation capabilities, as well as vimentin protein expression. Conclusion miR-141-3p can enhance the proliferation and migration of CNE-2 cells by promoting vimentin expression.