Can Micro RNA-24 Affect the Cardiovascular Morbidity in Systemic Lupus Erythematosus by Targeting YKL-40?

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2022-10-01 DOI:10.52547/rbmb.11.3.511
Maha Alhelf, Laila Rashed, Sahar Ahmed, Mohamed Mady, Marwa Abdelgwad
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Abstract

Background: Systemic lupus erythematosus (SLE) is an autoimmune disease with inflammatory nature. One of the leading causes of death in SLE patients is cardiovascular (CVS) morbidity. MiRNA-24 is highly expressed in vascular endothelial cells (VECs). This dysregulated expression pattern is associated with dysfunction or even damage of VECs and leads to the occurrence of cardiovascular diseases. YKL- 40 is an inflammatory glycoprotein involved in the pathogenesis of endothelial dysfunction and thereby atherosclerosis. In this work, we aimed at illustrating the possible role of miR-24 and its target YKL-40 in the pathogenesis of the CVS morbidity associated with SLE.

Methods: This work was conducted on 40 SLE patients and 40 healthy controls. Quantitative real-time PCR (qPCR) was done to estimate the expression level of miRNA-24 in serum. In addition, we measured the serum level of YKL-40 using ELISA.

Results: miR-24-fold change was found to be down-regulated, whereas serum YKL- 40 was up-regulated among SLE patients with observed significant and negative correlation between the two parameters.

Conclusion: Our study provided an insight about the role of miR-24 and its target serum YKL-40 protein in the development of SLE-related inflammation and atherosclerosis.

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微RNA-24能否通过靶向YKL-40影响系统性红斑狼疮患者心血管疾病的发生?
背景:系统性红斑狼疮(SLE)是一种具有炎症性的自身免疫性疾病。SLE患者死亡的主要原因之一是心血管疾病(CVS)的发病率。MiRNA-24在血管内皮细胞(VECs)中高度表达。这种失调的表达模式与VECs功能障碍甚至损伤有关,并导致心血管疾病的发生。YKL- 40是一种炎性糖蛋白,参与内皮功能障碍的发病机制,从而参与动脉粥样硬化。在这项工作中,我们旨在阐明miR-24及其靶点YKL-40在SLE相关CVS发病机制中的可能作用。方法:选取40例SLE患者和40例健康对照。采用实时荧光定量PCR (qPCR)检测血清中miRNA-24的表达水平。采用ELISA法检测血清YKL-40水平。结果:SLE患者血清mir -24倍变化下调,血清YKL- 40上调,两者呈显著负相关。结论:我们的研究揭示了miR-24及其靶血清YKL-40蛋白在sle相关炎症和动脉粥样硬化发生中的作用。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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