Ramzi Amin, Muhammad Apriliandy Shariff, Petty Purwanita, Mgs Irsan Saleh
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引用次数: 1
Abstract
Background: Diabetic retinopathy (DR) is one of diabetes mellitus complication and occurred in retinal microvascular. This study was aimed to investigate the efficacy of Sambiloto, Andrographis paniculate (A. paniculata) extract on glycemic profile, antioxidant and inflammatory cytokine parameters in diabetic rats, and phytochemical analysis of A. paniculata.
Methods: A. paniculata extract (APE) was carried out by maceration with ethanol. Diabetes mellitus in Wistar male rats was induced with streptozotocin. Retinal vessel diameters were estimated using a method by Vucetic. Inflammatory cytokine and antioxidant parameters were evaluated in retinal tissue. The alkaloid and flavonoid contents in extract were analyzed using thin layer chromatography method.
Results: Funduscopic examination presented some changes in the diameter of the blood vessels. The vessel diameter in the diabetic retinopathy group with APE in concentration of 100 and 200 mg/kg BW groups was significantly lower than in the DR group (p<0.05). The administration of APE in dosages of 100 and 200 mg/kg BW showed reduced glutathione, SOD, and catalase levels compared to the DR group (p<0.05).
Conclusion: A. paniculata extract doses of 100 and 200 mg/kg BW improved diabetic retinopathy in rats through hypoglycemic effects, antioxidant effects, and anti-inflammatory mechanisms.
背景:糖尿病视网膜病变(DR)是糖尿病的并发症之一，多发于视网膜微血管。本研究旨在探讨三比乐、穿心莲提取物对糖尿病大鼠血糖、抗氧化和炎症细胞因子的影响，并对穿心莲的植物化学成分进行分析。方法:用乙醇浸渍法提取穿心莲提取物。用链脲佐菌素诱导Wistar雄性大鼠糖尿病。使用Vucetic方法估计视网膜血管直径。观察视网膜组织的炎症因子和抗氧化参数。采用薄层色谱法对提取液中生物碱和类黄酮含量进行了分析。结果:眼底镜检查血管直径有一定变化。容器直径在糖尿病视网膜病变组与猿在100和200毫克/公斤体重组的浓度明显低于博士组(pConclusion: a .香提取剂100和200毫克/公斤体重改善糖尿病性视网膜病变的老鼠通过降糖效果,抗氧化效果,和抗炎机制。
期刊介绍：
The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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