A high-temperature sensitivity of Synechococcus elongatus PCC 7942 due to a tRNA-Leu mutation.

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2023-12-05 Epub Date: 2023-02-17 DOI:10.2323/jgam.2023.02.001
Hazuki Hasegawa, Yu Kanesaki, Satoru Watanabe, Kan Tanaka
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Abstract

Certain mutations of the model cyanobacterium Synechococcus elongatus PCC 7942 during laboratory storage have resulted in some divergent phenotypes. One laboratory-stored strain (H1) shows a temperature-sensitive (ts) growth phenotype at 40 °C. Here, we investigated the reason for this temperature sensitivity. Whole genome sequencing of H1 identified a single nucleotide mutation in synpcc7942_R0040 encoding tRNA-Leu(CAA). The mutation decreases the length of the tRNA-Leu t-arm from 5 to 4 base pairs, and this explains the ts phenotype. Secondary mutations suppressing the ts phenotype were identified in synpcc7942_1640, which putatively encodes a NYN domain-containing protein (nynA). The NYN domain is thought to be involved in tRNA/rRNA degradation. Thus, the structural stability of tRNA-Leu is critical for growth at 40 °C in Synechococcus elongatus PCC 7942.

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长聚球菌PCC 7942因tRNA-Leu突变而对高温敏感。
模型蓝藻长聚球菌PCC 7942在实验室储存期间的某些突变导致了一些不同的表型。一个实验室储存的菌株(H1)在40°C时显示出温度敏感(ts)的生长表型。在这里,我们研究了这种温度敏感性的原因。H1全基因组测序鉴定出编码tRNA-Leu(CAA)的synpcc7942_R0040单核苷酸突变。突变将tRNA-Leu t臂的长度从5个碱基对减少到4个碱基对,这解释了ts表型。在synpcc7942_1640中发现了抑制ts表型的继发性突变,该突变可能编码含有NYN结构域的蛋白(nynA)。NYN结构域被认为参与tRNA/rRNA降解。因此,tRNA-Leu的结构稳定性对长聚球菌PCC 7942在40°C下的生长至关重要。
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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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