The Effect of Vitamin D3 Supplemented with PhytoSolve on Genes Involved in Implantation in an NMRI Mice Model of Polycystic Ovary Syndrome: An Experimental Study.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Jornal Brasileiro de Reproducao Assistida Pub Date : 2023-11-14 DOI:10.5935/1518-0557.20230064
Sahar Hakimpour, Azam Govahi, Sahar Eghbali, Ronak Shabani, Rana Mehdizadeh, Marziyeh Ajdary, Mehdi Mehdizadeh
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Abstract

Objective: Vitamin D3 has been shown to be effective in the treatment of PCOS. However, due to its poor solvability and bioavailability, effective time is delayed and dosage requirements are increased. In our previous study, we demonstrated that PhytoSolve containing VD3 is more effective than vitamin D3 alone in the treatment of PCOS. In this study, we aimed to investigate the effect of this vitamin D3 formulation on gene expression involved in implantation in patients with PCOS.

Methods: To create PhytoSolve, Lipid S75, glycerol, and MCT oil were combined using a sonicator probe. Six groups, each consisting of 36 female Naval Medical Research Institute (NMRI) mice, were included in the following groups: control; sham; PCOS; PhytoSolve; PhytoSolve containing VD3; and vitamin D3. The mice were given DHEA injections to induce PCOS. After administering PhytoSolve containing VD3 and vitamin D3 by gavage for one week from the 13th day of model creation, the female mice were mated and endometrial tissue was collected for analysis of LIF, β-integrin, and HOXA10 proteins and genes.

Results: Compared to the group receiving vitamin D3 alone, the group receiving PhytoSolve containing vitamin D3 showed a significant increase in the expression of LIF, β-integrin, and HOXA10 genes (p<0.05). Although there was an increase in the expression of β-integrin and HOXA10 proteins in the group given PhytoSolve containing vitamin D3 compared to the group given vitamin D3, this increase was not significant. However, the increase in LIF protein expression in the group given PhytoSolve containing vitamin D3 was significant when compared to the group given vitamin D3 (p<0.05).

Conclusions: The use of PhytoSolve containing vitamin D3 was more effective than vitamin D3 alone. The PhytoSolve formulation might be a useful solution for medications with limited solubility and bioavailability.

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维生素D3补充PhytoSolve对多囊卵巢综合征NMRI小鼠模型着床相关基因影响的实验研究。
目的:维生素D3已被证明是有效的治疗多囊卵巢综合征。但由于其溶解性和生物利用度较差,延迟了有效时间,增加了用量要求。在我们之前的研究中,我们证明了含有VD3的PhytoSolve在治疗PCOS方面比单独的维生素D3更有效。在这项研究中,我们旨在研究这种维生素D3制剂对PCOS患者植入相关基因表达的影响。方法:将脂质S75、甘油和MCT油用声纳探针结合制备PhytoSolve。6组,每组36只雌性海军医学研究所(NMRI)小鼠,分为:对照组;虚假的;多囊卵巢综合征;PhytoSolve;含有VD3的PhytoSolve;和维生素D3小鼠注射脱氢表雄酮诱导多囊卵巢综合征。从造模第13天起灌胃含有VD3和维生素D3的PhytoSolve 1周后,雌性小鼠交配,收集子宫内膜组织,分析LIF、β-整合素和HOXA10蛋白和基因。结果:与单独服用维生素D3组相比,服用含有维生素D3的PhytoSolve组LIF、β-整合素和HOXA10基因的表达显著增加(结论:使用含有维生素D3的PhytoSolve比单独服用维生素D3更有效)。PhytoSolve制剂可能是一种有用的解决方案与有限的溶解度和生物利用度的药物。
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来源期刊
CiteScore
3.30
自引率
6.70%
发文量
56
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