The Cytotoxicity of Dental Restorative Materials on Gingival Stromal Mesenchymal Cells-an In Vitro Study.

Current Health Sciences Journal Pub Date : 2022-07-01 Epub Date: 2022-09-30 DOI:10.12865/CHSJ.48.03.12
Diana Oneț, Alexandra Roman, Andrada Soancă, Andreea Ciurea, Iulia Cristina Micu, Ștefan Cristian Vesa, Dora-Maria Popescu, Ana-Maria Rîcă
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Abstract

Background: Due to their minimally invasive high-quality adhesive, aesthetic and mechanical qualities, composite resins are the most frequently used materials in modern restorative dentistry. However, polymerization shrinkage and cytotoxicity are still unresolved drawbacks associated with these biomaterials.

Purpose: The present study aimed to assess the cytotoxicity of some restorative resin-based materials on gingival mesenchymal stromal cells (gMSCs), assuming that no differences in their behavior will be highlighted.

Material and methods: The cytotoxicity of the tested materials was evaluated by comparing the behavior of gMSCs cultured in normal conditions and in association with disc-shaped material samples indirectly through functionality tests (colony-forming unit-fibroblast assay, migratory potential) and directly through the MTT assay. The results were statistically analyzed with the ANOVA test and Tukey's Honest Significant Difference test.

Results: According to the MTT assay, there are no statistically significant differences regarding the viability of gMSCs cultured in normal conditions or in the presence of resin-based material samples. On the other hand, the present study identified a significantly reduced number of colonies formed by the gMSCs cultured in association with BF and B discs, compared to that of gMSCs cultured in normal conditions. Also, the migratory potential was significantly lower for control gMSCs when compared to ZE-gMSCs and significantly higher for ZE-gMSCs when compared to BF-gMSCs or BFL-gMSCs.

Conclusions: The results of the present study highlight a possible risk of cytotoxicity when using resin based-materials in dental practice, but they cannot be directly extrapolated to in vivo situations.

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口腔修复材料对牙龈基质间充质细胞的细胞毒性体外研究。
背景:复合树脂具有微创、高质量的粘合剂、美观和机械性能,是现代口腔修复中最常用的材料。然而,聚合收缩和细胞毒性仍然是与这些生物材料相关的未解决的缺点。目的:本研究旨在评估一些修复性树脂基材料对牙龈间充质基质细胞(gMSCs)的细胞毒性,假设它们的行为没有差异。材料和方法:通过比较在正常条件下培养的gMSCs的行为,并通过功能测试(集落形成单位成纤维细胞测定、迁移潜力)和直接通过MTT测定间接与盘状材料样品相结合,来评估受试材料的细胞毒性。采用方差分析和Tukey诚实显著性差异检验对结果进行统计学分析。结果:根据MTT测定,在正常条件下或在树脂基材料样品存在下培养的gMSCs的生存能力没有统计学上的显著差异。另一方面,本研究发现,与正常条件下培养的gMSC相比,与BF和B椎间盘联合培养的gMSCs形成的集落数量显著减少。此外,与ZE-gMSCs相比,对照gMSCs的迁移潜力明显较低,而与BF gMSCs或BFL-gMSCs相比ZE-gMSC的迁移潜力显著较高。结论:本研究的结果强调了在牙科实践中使用树脂基材料时可能存在细胞毒性风险,但不能直接推断为体内情况。
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