Diagnostic performance of lactate dehydrogenase (LDH) isoenzymes levels for the severity of COVID-19.

IF 1.5 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Medical Biochemistry Pub Date : 2023-01-20 DOI:10.5937/jomb0-37234

Ilkay Ergenc, Emre Capar, Sengel Buket Erturk, Gunel Bahramzade, Fatih Atalah, Derya Kocakaya, Sait Karakurt, Goncagul Haklar, Zekaver Odabasi

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引用次数: 2

Abstract

Background: Lactate dehydrogenase (LDH) levels predict coronavirus disease 2019 (COVID-19) severity. We investigated LDH isoenzyme levels to identify the tissue responsible for serum LDH elevation in patients with COVID-19.

Methods: Hospitalised COVID-19 patients with serum LDH levels exceeding the upper reference limit included. LDH isoenzymes were detected quantitatively on agarose gels. The radiological severity of lung involvement on computed tomography was scored as 0-5 for each lobe (total possible score, 0-25). Disease severity was determined using the World Health Organization (WHO) clinical progression scale.

Results: In total, 111 patients (mean age, 59.96 ± 16.14), including 43 females (38.7%), were enrolled. The serum levels of total LDH and all five LDH isoenzymes were significantly higher in the severe group. The levels of all LDH isoenzymes excluding LDH5 positively correlated with the WHO score. LDH3 levels correlated with chest computed tomography findings (r² = 0.267, p = 0.005). On multivariate analysis, LDH3 was an independent risk factor for the deterioration of COVID-19.

Conclusions: LDH3 appears to be an independent risk factor for deterioration in patients with COVID-19. LDH elevation in patients with COVID-19 predominantly resulted from lung, liver and muscle damage.

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乳酸脱氢酶(LDH)同工酶水平对COVID-19严重程度的诊断价值

背景:乳酸脱氢酶(LDH)水平可预测2019冠状病毒病(COVID-19)的严重程度。我们研究了LDH同工酶水平，以确定导致COVID-19患者血清LDH升高的组织。方法:纳入血清LDH水平超过参考上限的住院COVID-19患者。琼脂糖凝胶定量检测LDH同工酶。计算机断层扫描肺受累的放射学严重程度评分为0-5分(可能的总评分为0-25分)。采用世界卫生组织(WHO)临床进展量表确定疾病严重程度。结果:共纳入111例患者，平均年龄59.96±16.14岁，其中女性43例(38.7%)。重度组血清总LDH及5种LDH同工酶水平均显著升高。除LDH5外的所有LDH同工酶水平与WHO评分呈正相关。LDH3水平与胸部计算机断层扫描结果相关(r2 = 0.267, p = 0.005)。多因素分析显示，LDH3是COVID-19恶化的独立危险因素。结论:LDH3可能是COVID-19患者病情恶化的独立危险因素。COVID-19患者的LDH升高主要由肺、肝和肌肉损伤引起。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

12.00%

发文量

审稿时长

>12 weeks

期刊介绍： The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.