Effectiveness Of Pcr Pool Testing For Screening Of Covid-19 Infection In Pakistan.

Q3 Medicine Journal of Ayub Medical College, Abbottabad : JAMC Pub Date : 2022-10-01 DOI:10.55519/JAMC-04-10294

Nasir Ahmad, Hafsah Muhammad, Ishaq N Khan, Sami Siraj, Sajjad Ahmad, Muhammad Zakria, Asif Ali, Yasar Mehmood Yousafzai

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Abstract

Background: We tested the utility of mini-pool PCR testing for the rational use of PCR consumables in screening for CoViD-19.

Methods: After pilot experiments, 3-samples pool size was selected. One step RT-PCR was performed. The samples in the mini-pool having COVID gene amplification were tested individually.

Results: 1548 samples tested in 516 mini-pools resulted 396 mini-pools as negative and 120 as positive. Upon individual testing, 110 samples tested positive and 9 were inconclusive. 876 PCR reactions were performed to test 1548 samples, saving 43% PCR reagents. Centres with low prevalence resulted in most saving on reagents (50%), while centres with high prevalence resulted in more test reactions. Testing of individual samples resulted in delays in reporting.

Conclusions: Pooling can increase lab capacity, however, pooling delays results and cause degradation of samples.

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Pcr池检测在巴基斯坦筛查Covid-19感染的有效性

背景:我们检验了迷你池PCR检测在CoViD-19筛查中合理使用PCR耗材的效用。方法:经过中试，选取3个样本量。进行一步RT-PCR。对小型池中具有COVID基因扩增的样本进行单独检测。结果:516个小池中1548个样本检测，396个小池为阴性，120个为阳性。经个别检测，110个样本呈阳性，9个样本不确定。共进行876次PCR反应，检测1548份样品，节省43% PCR试剂。低流行率的中心节省了大部分试剂(50%)，而高流行率的中心节省了更多的测试反应。个别样品的测试导致报告延迟。结论:池化可以增加实验室容量，然而，池化延迟结果并导致样品降解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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