Synergistic Anti-Cancer Activity of the Combination of 1,25-Dihydroxyvitamin D3 and Retinoic Acid in U937 Cell Line.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2022-10-01 DOI:10.52547/rbmb.11.3.440
Abdollah Davodian, Kobra Foroughi, Amir Atashi, Masoud Soleiman
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Abstract

Background: MicroRNA is a form of non-coding RNAs that able to regulate gene expression. miR-424 is one of the members of the regulatory family, which plays an important role in the proliferation and differentiation of myeloid cells. Epigenetic changes can change the level of miR-424 under environmental factors. Therefore, the level of expression of miR-424 in U937 cells of the myeloid line was evaluated in this research under the influence of vitamin D3 (VitD3) and retinoic acid (RA).

Methods: In this study, U937 cells were cultured in the presence of VitD3, and RA to evaluate cell proliferation, viability via the trypan blue exclusion test, and expression level of miR-424 by real-time PCR at specific times.

Results: Cell proliferation has shown a significant decrease in the RA group versus other groups during incubation times (P < 0.05). In VitD3 group, there was a significant increase in cell proliferation after 24- and 48-hours incubation periods versus other groups. In the VitD3 and RA groups, the increase of cell proliferation caused the downregulation of miR-424. In addition, the upregulation of VitD3 group and downregulation of the RA group were significant versus the control group (P < 0.05).

Discussion: We concluded that the expression level of miR-424 was critically affected in the dose- and time-dependent of RA and VitD3 treatment in the U937 cell line. Treatment with VitD3 decreased the expression of miR-424 and RA treatment increase miR-424 expression level in physiological doses.

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1,25-二羟基维生素D3与维甲酸联合对U937细胞的协同抗癌作用。
背景:MicroRNA是一种能够调控基因表达的非编码rna。miR-424是调控家族成员之一,在髓细胞的增殖和分化中起重要作用。环境因素下表观遗传变化可改变miR-424的水平。因此,本研究在维生素D3 (VitD3)和维甲酸(RA)的影响下,评估miR-424在髓系U937细胞中的表达水平。方法:本研究将U937细胞在VitD3和RA存在下培养,通过台盼蓝排斥试验评估细胞增殖和活力,通过real-time PCR评估特定时间miR-424的表达水平。结果:RA组细胞增殖能力在孵育时间内明显低于其他各组(P < 0.05)。与其他组相比,VitD3组在孵育24小时和48小时后细胞增殖显著增加。在VitD3和RA组中,细胞增殖的增加导致miR-424的下调。此外,与对照组相比,VitD3组的上调和RA组的下调均有显著性差异(P < 0.05)。讨论:我们得出结论,在U937细胞系中,miR-424的表达水平在RA和VitD3治疗的剂量和时间依赖性中受到严重影响。VitD3治疗降低miR-424的表达,RA治疗在生理剂量下增加miR-424的表达水平。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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