Identification of Differential Metabolites Between  Type 2 Diabetes and Postchronic Pancreatitis Diabetes (Type 3c) Based on an Untargeted Metabolomics Approach.

Laboratory medicine Pub Date : 2023-11-02 DOI:10.1093/labmed/lmad004

Liang Qi, Zheng Ye, Hao Lin

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Abstract

Objective: A nontargeted metabolomics approach was established to characterize serum metabolic profile in type 3c diabetes mellitus (T3cDM) secondary to chronic pancreatitis and compare with T2DM.

Methods: Forty patients were recruited for metabolite analysis based on liquid chromatography-mass spectrometry. Cluster heatmap and KEGG metabolic pathway enrichment analysis were used to analyze the specific and differential metabolites. The receiver operating characteristics (ROCs) were generated and correlation analysis with clinical data was conducted.

Results: Metabolites including sphingosine, lipids, carnitine, bile acid, and hippuric acid were found to be different between T2DM and T3cDM, mainly enriched in bile acid biosynthesis, fatty acid biosynthesis, and sphingolipid metabolic pathways. The ROCs were generated with an area under the curve of 0.907 (95% confidence interval, 0.726-1) for the model with 15 metabolites.

Conclusion: T3cDM is characterized by increased sphingosine, carnitine, bile acid, and most lipids, providing novel biomarkers for clinical diagnosis and a future direction in research on pathophysiological mechanisms.

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基于非靶向代谢组学方法鉴定 2型糖尿病和慢性胰腺炎后糖尿病(3c型)之间的差异代谢物

目的：建立一种非靶向代谢组学方法来表征慢性胰腺炎继发的3c型糖尿病（T3cDM）的血清代谢谱，并与T2DM进行比较。聚类热图和KEGG代谢途径富集分析用于分析特异性和差异性代谢产物。生成受试者操作特征（ROC），并进行与临床数据的相关性分析。结果：代谢产物包括鞘氨醇、脂质、肉碱、胆汁酸和马尿酸在T2DM和T3cDM之间存在差异，主要富集于胆汁酸生物合成、脂肪酸生物合成和鞘脂代谢途径。对于含有15种代谢物的模型，ROC的曲线下面积为0.907（95%置信区间，0.726-1）。结论：T3cDM具有鞘氨醇、肉毒碱、胆汁酸和大部分脂质增加的特征，为临床诊断提供了新的生物标志物，为病理生理机制的研究提供了未来的方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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