Marija Vasić, Aleksandra Topić, Bojan Marković, Neda Milinković, Evica Dinčić
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引用次数: 2
Abstract
Background: Multiple sclerosis (MS) is characterized by inflammation, demyelination and axonal degeneration. Oxidative stress (OS) plays a significant role in the pathogenesis of the disease. The aim of the study was to examine the association between OS and smoking on the MS development.
Methods: The study included 175 patients with relapsing-remitting multiple sclerosis (RRMS) (76 males, 99 females) and 254 healthy subjects (81 males and 173 females). Oxidative stress biomarkers in serum, Total Antioxidant Status (TAS) and Total Oxidative Status (TOS) were determined spectrophotometrically. Oxidative Stress Index (OSI) was calculated as the ratio of TOS and TAS. Urinary 8-oxo7,8-dihydro-2'-deoxyguanosine were determined by HPLC-MS/MS and expressed as 8-oxodG/creatinine.
Results: In females with RRMS were higher TOS, OSI and 8-oxodG/creatinine than in females in control group. The group of males with RRMS had lower level of TAS than the males in control group. Higher levels of 8-oxodG/creatinine was obtained in active, passive and former smokers with RRMS than in control group with the same exposition to tobacco smoke. Independent predictors of MS are passive smoking, increased OSI and increased levels of urinary 8-oxodG/creatinine.
Conclusions: Our results demonstrate that the OS parameters should be included in the assessment of the risk for MS development. Due to the more sensitivity to oxidative stress, females may be at higher risk of MS development. This data indicates the importance of introducing the antioxidant therapy as a complementary treatment in patients with RRMS.
背景:多发性硬化症(MS)以炎症、脱髓鞘和轴突变性为特征。氧化应激(OS)在该疾病的发病机制中起重要作用。这项研究的目的是检查多发性硬化症和吸烟对多发性硬化症发展的关系。方法:研究对象为175例复发-缓解型多发性硬化症(RRMS)患者(男性76例，女性99例)和254例健康受试者(男性81例，女性173例)。采用分光光度法测定血清氧化应激生物标志物、总抗氧化状态(TAS)和总氧化状态(TOS)。氧化应激指数(OSI)计算为TOS与TAS的比值。采用HPLC-MS/MS法测定尿8-氧- 7,8-二氢-2′-脱氧鸟苷，并以8-氧- g /肌酐表达。结果:RRMS患者TOS、OSI、8-oxodG/肌酐均高于对照组。RRMS男性组TAS水平低于对照组。主动吸烟者、被动吸烟者和RRMS前吸烟者的8-oxodG/肌酐水平均高于接触相同烟草烟雾的对照组。MS的独立预测因子是被动吸烟、OSI升高和尿8-氧合g /肌酐水平升高。结论:我们的研究结果表明，应将OS参数纳入MS发展风险的评估中。由于对氧化应激更敏感，女性发生多发性硬化症的风险更高。这一数据表明，在RRMS患者中引入抗氧化治疗作为补充治疗的重要性。
期刊介绍：
The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly.
The Journal publishes original scientific and specialized articles on all aspects of
clinical and medical biochemistry,
molecular medicine,
clinical hematology and coagulation,
clinical immunology and autoimmunity,
clinical microbiology,
virology,
clinical genomics and molecular biology,
genetic epidemiology,
drug measurement,
evaluation of diagnostic markers,
new reagents and laboratory equipment,
reference materials and methods,
reference values,
laboratory organization,
automation,
quality control,
clinical metrology,
all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.
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