Enzyme-Assisted Extraction of Narirutin from Citri Reticulatae Pericarpium and Anti-allergic Asthma Activity.

IF 1.1 4区医学 Q4 IMMUNOLOGY Iranian Journal of Immunology Pub Date : 2022-12-01 DOI:10.22034/IJI.2022.91640.2095

Xiaolei Shi, Lina Zhao, Liyan Niu, Jihao Wei, Xuwen Li

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Abstract

Background: Asthma is a heterogeneous disorder of the airways related to inflammation; it affects millions of people worldwide. Due to the side effects of inhaled corticosteroids, researchers focused on the therapeutic effects of compounds derived from natural products.

Objective: To investigate the therapeutic benefits of Narirutin a valuable flavonoid in Citri Reticulatae Pericarpium for asthma.

Methods: Narirutin was extracted using the enzyme-assisted method with the L9 (34) orthogonal array to optimize the temperatures, pH, and reaction time. The mechanism of action of Narirutin was investigated via ELISA, flow cytometry, and Western blot analysis in vivo.

Results: Narirutin suppressed inflammatory cell infiltration in the lung tissue and decreased IgE and IgG1 levels in serum in vivo. It can also alleviate interleukin (IL)-4, IL-5, and interferon-γ concentrations in bronchoalveolar lavage fluid in mice. Moreover, it increased the ratio of CD4+/CD8+ T cells. Additionally, Narirutin significantly suppressed p-ERK1/2 and p-JNK expression in the MAPK signaling pathway.

Conclusion: Narirutin affects the Th1/Th2 imbalance through the p-ERK and p-JNK suppression in the MAPK signaling pathway.

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柑橘皮中纳瑞素的酶辅助提取及抗过敏性哮喘活性研究。

背景:哮喘是一种与炎症相关的气道异质性疾病;它影响着全世界数百万人。由于吸入皮质类固醇的副作用，研究人员专注于从天然产物中提取的化合物的治疗效果。目的:探讨柑桔皮中有价值的类黄酮纳瑞鲁丁对哮喘的治疗作用。方法:采用L9(34)正交设计优化提取温度、pH、反应时间，采用酶助提取法提取纳瑞芦丁。采用酶联免疫吸附测定、流式细胞术、Western blot等方法对纳瑞鲁丁的作用机制进行了研究。结果:纳瑞鲁丁在体内抑制肺组织炎症细胞浸润，降低血清IgE和IgG1水平。它还能降低小鼠支气管肺泡灌洗液中白细胞介素(IL)-4、IL-5和干扰素-γ浓度。CD4+/CD8+ T细胞比值升高。此外，Narirutin显著抑制MAPK信号通路中p-ERK1/2和p-JNK的表达。结论:Narirutin通过抑制MAPK信号通路中的p-ERK和p-JNK影响Th1/Th2失衡。

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来源期刊

Iranian Journal of Immunology Medicine-Immunology and Allergy

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Immunology (I.J.I) is an internationally disseminated peer-reviewed publication and publishes a broad range of experimental and theoretical studies concerned with all aspects of immunology.