Gingival crevicular fluid CSF-1 and IL-34 levels in patients with stage III grade C periodontitis and uncontrolled type 2 diabetes mellitus.

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Periodontal and Implant Science Pub Date : 2022-12-01 Epub Date: 2022-04-12 DOI:10.5051/jpis.2106260313

Ahu Dikilitaş, Fatih Karaaslan, Şehrazat Evirgen, Abdullah Seçkin Ertuğrul

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Abstract

Purpose: Periodontal diseases are inflammatory conditions that alter the host's response to microbial pathogens. Type 2 diabetes mellitus (T2DM) is a complex disease that affects the incidence and severity of periodontal diseases. This study investigated the gingival crevicular fluid (GCF) levels of colony-stimulating factor-1 (CSF-1) and interleukin-34 (IL-34) in patients with stage III grade C periodontitis (SIII-GC-P) and stage III grade C periodontitis with uncontrolled type 2 diabetes (SIII-GC-PD).

Methods: In total, 72 individuals, including 24 periodontally healthy (PH), 24 SIII-GC-P, and 24 SIII-GC-PD patients, were recruited for this study. Periodontitis patients (stage III) had interdental attachment loss (AL) of 5 mm or more, probing depth (PD) of 6 mm or more, radiographic bone loss advancing to the middle or apical part of the root, and tooth loss (<5) due to periodontal disease. Radiographic bone loss in the teeth was also evaluated; grade C periodontitis was defined as a ratio of the percentage of root bone loss to age greater than 1.0. The plaque index (PI), gingival index (GI), presence of bleeding on probing (BOP), PD, and clinical AL were used for clinical periodontal assessments. GCF samples were obtained and analyzed using an enzyme-linked immunosorbent assay.

Results: All clinical parameters-PD, AL, GI, BOP, and PI-were significantly higher in the SIII-GC-PD group than in the PH and SIII-GC-P groups for both the full mouth and each sampling site (P<0.05). The total IL-34 and CSF-1 levels were significantly higher in the SIII-GC-PD group than in the PH and SIII-GC-P groups (P<0.05), and there were significant differences between the periodontitis groups (P<0.05).

Conclusions: These findings suggest that IL-34 and CSF-1 expression increases in patients with SIII-GC-PD. CSF-1 was associated with the inflammatory status of periodontal tissues and T2DM, while IL-34 was associated only with T2DM.

Trial registration: ClinicalTrials.gov Identifier: NCT04891627.

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III 期 C 级牙周炎和未控制的 2 型糖尿病患者的龈沟液 CSF-1 和 IL-34 水平。

目的：牙周疾病是一种炎症，会改变宿主对微生物病原体的反应。2 型糖尿病（T2DM）是一种复杂的疾病，会影响牙周疾病的发病率和严重程度。本研究调查了 III 期 C 级牙周炎（SIII-GC-P）和 III 期 C 级牙周炎伴未控制的 2 型糖尿病（SIII-GC-PD）患者牙龈缝隙液（GCF）中集落刺激因子-1（CSF-1）和白细胞介素-34（IL-34）的水平：本研究共招募了 72 人，包括 24 名牙周健康（PH）患者、24 名 SIII-GC-P 患者和 24 名 SIII-GC-PD 患者。牙周炎患者（III期）的牙间附着丧失（AL）达到或超过5毫米，探诊深度（PD）达到或超过6毫米，影像学显示牙槽骨丧失向牙根中部或根尖部推进，以及牙齿脱落：在全口和每个取样部位，SIII-GC-PD 组的所有临床参数--PD、AL、GI、BOP 和 PI--均显著高于 PH 组和 SIII-GC-P 组（PPPConclusions：这些研究结果表明，IL-34 和 CSF-1 在 SIII-GC-PD 患者中表达增加。CSF-1 与牙周组织的炎症状态和 T2DM 相关，而 IL-34 仅与 T2DM 相关：试验注册：ClinicalTrials.gov Identifier：试验注册：ClinicalTrials.gov Identifier：NCT04891627。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Periodontal and Implant Science DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.30%

发文量

期刊介绍： Journal of Periodontal & Implant Science (JPIS) is a peer-reviewed and open-access journal providing up-to-date information relevant to professionalism of periodontology and dental implantology. JPIS is dedicated to global and extensive publication which includes evidence-based original articles, and fundamental reviews in order to cover a variety of interests in the field of periodontal as well as implant science.