Molecular isolation and expression analysis of hemocyanin isoform 2 of Macrobrachium rosenbergii

IF 1.5 4区 农林科学 Q3 FISHERIES Journal of aquatic animal health Pub Date : 2022-10-07 DOI:10.1002/aah.10173
Chutima Srisuk, Chanitcha Choolert, William G. Bendena, Siwaporn Longyant, Paisarn Sithigorngul, Parin Chaivisuthangkura
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罗氏沼虾血青素异构体2的分子分离与表达分析
目的:血青素是许多节肢动物和软体动物血淋巴中的含铜蛋白,是一种重要的氧转运蛋白和非特异性免疫蛋白。方法:采用快速扩增的方法分离罗氏沼虾(Macrobrachium rosenbergii)血青素异构体2 (MrHc2)的互补DNA,并对其mRNA表达进行表征,阐明其功能的分子基础。结果:MrHc2分子质量为77.3 kDa,包含三个结构域:全α血青素、含铜血青素和免疫球蛋白样血青素结构域。分子系统发育分析表明,MrHc2属于γ型亚基,与日本沼虾(Macrobrachium nipponense)的血青素亚基1密切相关。此外,MrHc2与罗氏m.r obergii (α型亚基)的血青素(MrHc)和对虾的血青素蛋白处于不同的分支。MrHc2信使RNA转录本在肝胰腺中高表达,在鳃、肠、胃、肌肉和血细胞中弱表达。罗森氏诺达病毒(MrNV)攻毒后,MrHc2的表达量在第3、4、5天分别为1.96倍、2.93倍和1.96倍,在第7天逐渐下降到基础水平。结论:本研究提示MrHc2在罗氏分枝杆菌对MrNV的先天免疫应答中起重要作用。
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来源期刊
Journal of aquatic animal health
Journal of aquatic animal health 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
25
审稿时长
>24 weeks
期刊介绍: The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.
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