Ayse Arikan, Osman Doluca, Sila Akhan, Tamer Sanlidag, Murat Sayan
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引用次数: 0
Abstract
Background: Antibody testing can complement molecular assays for detecting COVID-19.
Aims: We evaluated the concurrence between lateral flow assay and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Methods: The study was conducted at Kocaeli University, Türkiye. We used a lateral flow assay and ELISA to test serum samples from COVID-19 cases, confirmed by polymerase chain reaction assays (study group) and pre-pandemic stored serum samples (control group). We used Deming regression to evaluate the antibody measurements.
Results: The study group included 100 COVID-19 cases, and the control group included pre-pandemic samples from 156 individuals. The lateral flow assay detected immunoglobulin M (IgM) and G (IgG) antibodies in 35 and 37 study group samples. ELISA detected IgM nucleocapsid (N) antibodies in 18 samples, and IgG (N) and IgG spike 1 (S1) antibodies in 31 and 29 samples, respectively. None of the techniques detected antibodies in the control samples. Strong correlations were found between lateral flow IgG (N+ receptor-binding domain + S1) and ELISA IgG (S) (r = 0.93, P < 0.01) and ELISA IgG (N) (r = 0.81, P < 0.01). Weaker correlations were seen between ELISA IgG S and IgG N (r = 0.79, P < 0.01) and lateral flow assay and ELISA IgM (N) (r = 0.70, P < 0.01).
Conclusion: Lateral flow assay and ELISA techniques gave consistent results for IgG/IgM antibody measurements towards spike and nucleocapsid proteins, suggesting that both methods can be used to detect COVID-19 where access to molecular test kits is difficult.
期刊介绍：
The Eastern Mediterranean Health Journal, established in 1995, is the flagship health periodical of the World Health Organization Regional Office for the Eastern Mediterranean.
The mission of the Journal is to contribute to improving health in the Eastern Mediterranean Region by publishing and publicising quality health research and information with emphasis on public health and the strategic health priorities of the Region. It aims to: further public health knowledge, policy, practice and education; support health policy-makers, researchers and practitioners; and enable health professionals to remain informed of developments in public health.
The EMHJ:
-publishes original peer-reviewed research and reviews in all areas of public health of relevance to the Eastern Mediterranean Region
-encourages, in particular, research related to the regional health priorities, namely: health systems strengthening; emergency preparedness and response; communicable diseases; noncommunicable diseases and mental health; reproductive, maternal, child health and nutrition
-provides up-to-date information on public health developments with special reference to the Region.
The Journal addresses all members of the health profession, health educational institutes, as well as governmental and nongovernmental organizations in the area of public health within and outside the Region.
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