The methylation status of GATA3 potentially predicts the outcomes of assisted reproductive technologies.

IF 2.1 4区医学 Q2 OBSTETRICS & GYNECOLOGY Human Fertility Pub Date : 2023-12-01 Epub Date: 2023-01-10 DOI:10.1080/14647273.2023.2164871

Mina Beiraghdar, Mozhdeh Beiraghdar, Sharifeh Khosravi

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Abstract

Evaluation of methylation status of genes in sperm samples has been suggested for diagnosis of male infertility as well as prognosis of assisted reproductive technologies (ART) outcomes. In this study, we compared the methylation pattern of the GATA3 gene in infertile and fertile men as well as in infertile men with positive and negative ART outcome based on clinical pregnancy. Ejaculates were obtained from 42 infertile men with a negative ART outcome (group 1), 30 infertile men with a positive ART outcome (group 2), and 21 fertile men (control). Then, samples were subjected to genomic DNA isolation and subsequent TUNEL assay and methylation-specific PCR. The number of infertile men with at least one methylated allele of GATA3 was significantly higher compared to the control group (p = 0.022). Also, the number of patients with at least one methylated allele was significantly higher in group 1 compared to group 2 (p = 0.013). Moreover, the TUNEL assay revealed that the amount of sperm DNA fragmentation is higher in group 1 compared to group 2 (p = 0.008). The findings of our study demonstrated that the degree of GATA3 methylation can potentially differentiate between infertile and fertile men and more importantly can potentially predict the outcome of ART.

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GATA3 的甲基化状态可预测辅助生殖技术的结果。

有人建议评估精子样本中基因的甲基化状态，用于诊断男性不育症以及辅助生殖技术（ART）结果的预后。在这项研究中，我们比较了不育男性和可育男性以及根据临床妊娠结果判断为阳性和阴性 ART 结果的不育男性中 GATA3 基因的甲基化模式。研究人员从 42 名抗逆转录病毒疗法结果为阴性的不育男性（第 1 组）、30 名抗逆转录病毒疗法结果为阳性的不育男性（第 2 组）和 21 名可育男性（对照组）中获取了射精样本。然后，对样本进行基因组 DNA 分离、TUNEL 检测和甲基化特异性 PCR 检测。与对照组相比，不育男性中至少有一个 GATA3 甲基化等位基因的人数明显较多（p = 0.022）。此外，与第 2 组相比，第 1 组中至少有一个甲基化等位基因的患者人数明显较多（p = 0.013）。此外，TUNEL 检测显示，与第 2 组相比，第 1 组的精子 DNA 断裂量更高（p = 0.008）。我们的研究结果表明，GATA3 甲基化程度有可能区分不育和可育男性，更重要的是，它有可能预测抗逆转录病毒疗法的结果。

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来源期刊

Human Fertility OBSTETRICS & GYNECOLOGY-REPRODUCTIVE BIOLOGY

CiteScore

3.30

自引率

5.30%

发文量

期刊介绍： Human Fertility is a leading international, multidisciplinary journal dedicated to furthering research and promoting good practice in the areas of human fertility and infertility. Topics included span the range from molecular medicine to healthcare delivery, and contributions are welcomed from professionals and academics from the spectrum of disciplines concerned with human fertility. It is published on behalf of the British Fertility Society. The journal also provides a forum for the publication of peer-reviewed articles arising out of the activities of the Association of Biomedical Andrologists, the Association of Clinical Embryologists, the Association of Irish Clinical Embryologists, the British Andrology Society, the British Infertility Counselling Association, the Irish Fertility Society and the Royal College of Nursing Fertility Nurses Group. All submissions are welcome. Articles considered include original papers, reviews, policy statements, commentaries, debates, correspondence, and reports of sessions at meetings. The journal also publishes refereed abstracts from the meetings of the constituent organizations.