A positive feedback loop of heparanase/syndecan1/nerve growth factor regulates cancer pain progression.

IF 3.4 3区医学 Q2 CLINICAL NEUROLOGY Korean Journal of Pain Pub Date : 2023-01-01 DOI:10.3344/kjp.22277

Xiaohu Su, Bingwu Wang, Zhaoyun Zhou, Zixian Li, Song Tong, Simin Chen, Nan Zhang, Su Liu, Maoyin Zhang

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引用次数: 1

Abstract

Background: The purpose of this research was to assess the role of heparanase (HPSE)/syndecan1 (SDC1)/nerve growth factor (NGF) on cancer pain from melanoma.

Methods: The influence of HPSE on the biological function of melanoma cells and cancer pain in a mouse model was evaluated. Immunohistochemical staining was used to analyze HPSE and SDC1. HPSE, NGF, and SDC1 were detected using western blot. Inflammatory factors were detected using ELISA assay.

Results: HPSE promoted melanoma cell viability, proliferation, migration, invasion, and tumor growth, as well as cancer pain, while SST0001 treatment reversed the promoting effect of HPSE. HPSE up-regulated NGF, and NGF feedback promoted HPSE. High expression of NGF reversed the inhibitory effect of HPSE down-regulation on melanoma cell phenotype deterioration, including cell viability, proliferation, migration, and invasion. SST0001 down-regulated SDC1 expression. SDC1 reversed the inhibitory effect of SST0001 on cancer pain.

Conclusions: The results showed that HPSE promoted melanoma development and cancer pain by interacting with NGF/SDC1. It provides new insights to better understand the role of HPSE in melanoma and also provides a new direction for cancer pain treatment.

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肝素酶/syndecan1/神经生长因子的正反馈循环调节癌性疼痛的进展。

背景:本研究的目的是评估肝素酶(HPSE)/syndecan1 (SDC1)/神经生长因子(NGF)在黑色素瘤癌痛中的作用。方法:观察HPSE对小鼠黑色素瘤细胞生物学功能和癌痛的影响。免疫组化染色检测HPSE和SDC1。western blot检测HPSE、NGF、SDC1。采用ELISA法检测炎症因子。结果:HPSE促进黑色素瘤细胞活力、增殖、迁移、侵袭和肿瘤生长，以及癌痛，而SST0001治疗逆转了HPSE的促进作用。HPSE上调NGF，而NGF反馈促进HPSE。NGF的高表达逆转了HPSE下调对黑色素瘤细胞表型恶化的抑制作用，包括细胞活力、增殖、迁移和侵袭。SST0001下调SDC1的表达。SDC1逆转了SST0001对癌痛的抑制作用。结论:结果表明HPSE通过与NGF/SDC1相互作用促进黑色素瘤的发展和癌性疼痛。这为更好地理解HPSE在黑色素瘤中的作用提供了新的见解，也为癌症疼痛治疗提供了新的方向。

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来源期刊

Korean Journal of Pain Medicine-Anesthesiology and Pain Medicine

CiteScore

5.40

自引率

7.10%

发文量

审稿时长

16 weeks

期刊介绍： Korean Journal of Pain (Korean J Pain, KJP) is the official journal of the Korean Pain Society, founded in 1986. It has been published since 1988. It publishes peer reviewed original articles related to all aspects of pain, including clinical and basic research, patient care, education, and health policy. It has been published quarterly in English since 2009 (on the first day of January, April, July, and October). In addition, it has also become the official journal of the International Spinal Pain Society since 2016. The mission of the Journal is to improve the care of patients in pain by providing a forum for clinical researchers, basic scientists, clinicians, and other health professionals. The circulation number per issue is 50.