Kisspeptin and kisspeptin receptor immunoreactivity in euploid and aneuploid choriodecidual tissues of recurrent pregnancy losses

Amr O. Abdelkareem M.B.B.Ch., M.D. , Sahar M. Gebril M.D. , Faten F. AbdelHafez M.D. , Jefferson Terry M.D., Ph.D. , Mohamed A. Bedaiwy M.D., Ph.D.
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引用次数: 3

Abstract

Objective

To study choriodecidual immunoreactivity of kisspeptin (KISS1) and its receptor (KISS1R) in recurrent pregnancy loss (RPL) due to aneuploidy (AnE) and unexplained (UE) RPL in comparison to control elective abortions (EAbs).

Design

This is a case-control study.

Setting

Tertiary care facility and affiliated research institute.

Patient(s)

Patients with either UE RPL (n = 10) or RPL due to AnE (n = 10) vs. a control group of patients who underwent EAb (n = 10).

Intervention(s)

Immunohistochemistry of archived choriodecidual tissue samples.

Main Outcome Measure(s)

Histoscores of KISS1 and KISS1R immunoreactivity in the syncytiotrophoblast (SyT), cytotrophoblast (CyT), decidual glands (DeGs), and decidual stroma (DeS) across the 3 study groups.

Result(s)

There was no difference in both maternal and gestational ages among the 3 groups. Kisspeptin immunoreactivity was similar in the SyT, CyT, DeGs, and DeS of all groups. Similarly, KISS1R expression was not different in the DeGs or DeS among all study groups. In addition, there was no difference in KISS1R immunoreactivity in the SyTs and CyTs between patients with RPL due to AnE and those with UE RPL. However, KISS1R was significantly lower in the SyT and CyT of patients with RPL due to AnE and UE RPL than in those who underwent EAb.

Conclusion(s)

The expression of KISS1R is lower in the chorionic tissues of euploid (unexplained) and aneuploid RPLs than in the control group. The current results broaden our understanding of the role played by KISS1 and KISS1R in early placentation. Further investigation is necessary to determine whether KISS1 activity is the cause or a sequel of defective placentation.

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复发性妊娠丢失的整倍体和非整倍体绒毛膜蜕膜组织中的Kisspeptin和Kisspeptin受体免疫反应性
目的研究kisspeptin (KISS1)及其受体(KISS1R)在非整倍体(AnE)和不明原因(UE)所致复发性妊娠丢失(RPL)中的绒毛膜蜕膜免疫反应性,并与对照选择性流产(EAbs)进行比较。设计本研究为病例对照研究。背景:三级医疗机构和附属研究机构。患者:患有UE RPL (n = 10)或因AnE引起RPL (n = 10)的患者与接受EAb治疗的对照组(n = 10)。干预措施:存档的绒毛膜蜕膜组织样本的免疫组化。主要观察指标(5)3个研究组大鼠合胞滋养细胞(SyT)、细胞滋养细胞(CyT)、蜕膜腺(DeGs)和蜕膜间质(DeS)中KISS1和KISS1R免疫反应性的组织评分。结果(5)3组大鼠母龄和胎龄均无差异。Kisspeptin免疫反应性在各组的SyT、CyT、deg和DeS中相似。同样,KISS1R在所有研究组的deg或de中的表达也没有差异。此外,AnE所致RPL患者与UE所致RPL患者的syt和cyt中KISS1R的免疫反应性无差异。然而,与接受eab治疗的RPL患者相比,AnE和UE RPL患者的SyT和CyT中KISS1R的表达明显降低。结论(5)整倍体(原因不明)和非整倍体RPL的绒毛膜组织中KISS1R的表达低于对照组。目前的结果拓宽了我们对KISS1和KISS1R在早期胎盘中所起作用的理解。需要进一步的研究来确定KISS1活性是胎盘缺陷的原因还是后果。
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来源期刊
F&S science
F&S science Endocrinology, Diabetes and Metabolism, Obstetrics, Gynecology and Women's Health, Urology
CiteScore
2.00
自引率
0.00%
发文量
0
审稿时长
51 days
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