Effect of black pepper, turmeric and ajwa date on the endocrine pancreas of the experimentally induced diabetes in wister albino rats: A histological and immunohistochemical study

Q3 Medicine Endocrine and Metabolic Science Pub Date : 2021-09-30 DOI:10.1016/j.endmts.2021.100098

Maliha Sarfraz , Tanweer Khaliq , Rahman M. Hafizur , Sayed Ali Raza , Hayat Ullah

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引用次数: 6

Abstract

Background

Diabetes is now a global problem and millions of people are suffering all over the world. Reports exist for the allopathic use of turmeric, black pepper, and date palm as an antidiabetic and antioxidant agent.

Aim

The current study was designed to assess the antihyperglycemic, antioxidants and antihyperlipidemic consequences of black pepper (BP), turmeric (T), ajwa pulp (AP), and ajwa seeds (AS) in alloxan-induced diabetic rats.

Methodology

Diabetes was induced by a single intraperitoneal injection of alloxan (150 mg/kg b.w) in rats. They were randomly divided into 11 groups of 18 male and 18 female rats each. Group-1 normal control, group-2 diabetic control, group-3 was administered with glibenclamide (10 mg/kg), group-4 was administered with aqueous extract of BP (50 mg/kg), group-5, 6, 7 were administered with T, AP and AS (500 mg/kg) and group-8, 9, 10, 11 were administered with different combinations of aqueous extract (500 mg/kg) once in a day for eight weeks. The antihyperglycemic potential was determined through biochemical and histological investigations of the experimental animals at the end of the experiment.

Results

The results of the study revealed that treatments improved glucose (229.53 mg/dL), Ghb (7.68%), insulin (13.63 U/L), Tg (95.92 mg/dL), Tc (152.86 mg/dL), HDL (23.22 mg/dL), LDL (110.30 mg/dL), TAC (1.89 mmol/L) and TOS (20.05 μmol/L) in comparison to diabetic control rats after 8 weeks of study period. Histological and immunohistochemical investigation of tissues exhibited severe changes in the pancreas of diabetic rats and treatments modulate these changes; this improvement in cells may explain the antidiabetic effects.

Conclusion

It is concluded that aqueous extract of BP+AS; and BP+T+AP+AS could be promising nutraceutical therapy for diabetes management and its associated complications.

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黑胡椒、姜黄和红枣对实验性糖尿病大鼠内分泌胰腺的影响:组织学和免疫组化研究

糖尿病现在是一个全球性问题，全世界有数百万人患有糖尿病。有报道称姜黄、黑胡椒和椰枣作为抗糖尿病和抗氧化剂的对抗疗法使用。目的本研究旨在评价黑胡椒(BP)、姜黄(T)、ajwa果肉(AP)和ajwa种子(AS)对四氧嘧啶诱导的糖尿病大鼠的降糖、抗氧化剂和降血脂作用。方法采用单次腹腔注射四氧嘧啶(150 mg/kg b.w)诱导大鼠糖尿病。它们被随机分为11组，每组18只公鼠和18只母鼠。1组正常对照组、2组糖尿病对照组、3组给予格列本脲(10 mg/kg)， 4组给予BP水提物(50 mg/kg)， 5、6、7组给予T、AP、AS (500 mg/kg)， 8、9、10、11组给予不同组合水提物(500 mg/kg)，每天1次，连用8周。实验结束时对实验动物进行生化和组织学检查，测定其降糖潜能。结果8周后，与糖尿病对照组相比，葡萄糖(229.53 mg/dL)、Ghb(7.68%)、胰岛素(13.63 U/L)、Tg (95.92 mg/dL)、Tc (152.86 mg/dL)、HDL (23.22 mg/dL)、LDL (110.30 mg/dL)、TAC (1.89 mmol/L)和TOS (20.05 μmol/L)均得到改善。对糖尿病大鼠胰腺组织的组织学和免疫组织化学调查显示，胰腺组织发生了严重的变化，治疗可以调节这些变化;细胞的这种改善可以解释其抗糖尿病作用。结论BP+AS水提物;BP+T+AP+AS可能是治疗糖尿病及其相关并发症的有前途的营养品疗法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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