pH-dependent temperature sensitivity of rat lens phosphofructokinase.

Investigative ophthalmology Pub Date : 1976-06-01
H M Cheng, L T Chylack
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Abstract

Rat lens phosphofructokinase (PFK) has been found to be cold-labile at acidic pH, even in the presence of sulfate and inorganic phosphate, two known positive effectors. The inactivation appears to be an irreversible process, but can be prevented by including ATP in the incubating media. The enzyme is relatively stable at pH 8.2 incubated at 0 to 4 degrees, 25 degrees, or 37 degrees C. in the absence of the effectors, but is extremely thermolabile if the pH is lowered to 7.30 or lower. The thermolability is counteracted by many effectors, among them sulfate and ATP are the most effective. The physiologic significance of PFK instability and effector protection in the lens are discussed.

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大鼠晶状体磷酸果糖激酶的ph依赖性温度敏感性。
大鼠晶态磷酸果糖激酶(PFK)已被发现在酸性pH下是冷不稳定的,即使在硫酸盐和无机磷酸盐这两种已知的积极效应物存在下也是如此。失活似乎是一个不可逆的过程,但可以通过在培养培养基中加入ATP来防止。在没有效应物的情况下,该酶在pH 8.2、0 ~ 4度、25度或37℃孵育时相对稳定,但如果pH降至7.30或更低,则具有极强的耐热性。热性被许多效应剂抵消,其中硫酸盐和ATP是最有效的。讨论了PFK失稳的生理意义和晶状体的效应保护。
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