Some factors affecting testosterone, dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3 beta,17 beta-diol secretion by invitro perfused rabbit testes.

Current topics in molecular endocrinology Pub Date : 1975-01-01

L L Ewing

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Abstract

Intra-arterial infusion of testosterone-3H gave rise to tritiated dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3beta,17 beta-diol in spermatic venous effluent of the perfused rabbit testis-epididymis. Mass spectrometric measurements confirmed that these four androgens were present in spermatic venous effluent of the perfused rabbit testis-epididymis. Gas liquid chromatographic measurement showed that testosterone, dihydrotestosterone, 5 alpha-androstan-3 alpha,17 beta-diol and 5 alpha-androstan-3 beta,17 beta-diol were secreted in similar amounts by the in vitro perfused and in situ rabbit testis-epididymis results obtained by perfusing the testis minus the epididymis suggested that the bulk of these androgens originate from the catabolism of testosterone within the testis rather than the epididymis. Suprisingly, germinal epithelium destruction by heat failed to alter the testosterone, dihydrotestosterone and 5 alpha-androstan-3 alpha,17 beta-diol secretion by the in vitro perfused rabbit testis. In contrast, the secretion of 5 alpha-androstan-3 beta,17 beta-diol was significantly (P less than 0.05) reduced in the same cryptorchid compared to control testes.

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体外灌注兔睾丸分泌睾酮、二氢睾酮、5 -雄激素-3 α、17 -二醇及5 -雄激素-3 β、17 -二醇的影响因素

动脉灌注睾酮- 3h后，兔睾丸附睾精静脉流出液中产生氚化的二氢睾酮、5 α -雄酮-3 α、17 β -二醇和5 α -雄酮-3 β、17 β -二醇。质谱测定证实，这四种雄激素存在于灌注兔睾丸-附睾的精静脉流出物中。气相色谱测定表明，体外灌注和原位灌注兔睾丸-附睾分泌的睾酮、二氢睾酮、5 α -雄激素-3 α、17 β -二醇和5 α -雄激素-3 β、17 β -二醇的量相似，通过灌注睾丸而非附睾获得的结果表明，这些雄激素的大部分来自睾丸内的睾酮分解代谢，而不是来自附睾。令人惊讶的是，热破坏生殖上皮并没有改变体外灌注兔睾丸的睾酮、二氢睾酮和5 α -雄激素-3 α，17 β -二醇的分泌。与对照组相比，5 α -雄激素-3 β、17 β -二醇的分泌量显著(P < 0.05)降低。

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Current topics in molecular endocrinology

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