Study of the lysosomes by vital stains in normal keratocytes and in keratocytes from macular dystrophy of the cornea.

Investigative ophthalmology Pub Date : 1976-08-01
J François, V Victoria-Troncoso, P C Maudgal, A Victoria-Ihler
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Abstract

The acridine orange is an excellent vital stain for the "in vivo" study of the lysosomal dynamics in cultured keratocytes. In normal keratocytes the lysosomes are mostly grouped around one or both nucleus poles. In keratocytes from macular dystrophy of the cornea there is a hypertrophy of the lysosomal system, which in vain tends to compensate for the consequences of the enzymatic deficiency.

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正常角膜细胞和黄斑性角膜营养不良的角膜细胞溶酶体的活体染色研究。
吖啶橙是一种极好的活体染色剂,用于在体内研究培养的角化细胞的溶酶体动力学。在正常的角化细胞中,溶酶体大多聚集在一个或两个核极周围。在角膜黄斑营养不良的角化细胞中,溶酶体系统肥大,这徒劳地倾向于补偿酶缺乏的后果。
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A new technique for the vital staining of the corneal endothelium. Niemann-Pick disease-like inclusions caused by a hypocholesteremic agent. Experimental disciform edema and necrotizing keratitis in the rabbit. Immunofluorescent studies on the trabecular meshwork in open-angle glaucoma. A randomized technique of constant-pressure infusion.
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