{"title":"[Studies of acid-soluble antigens in several strains of the genus Bacillus for orienting purposes (author's transl)].","authors":"K Katsaras, E Hellmann","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>By acid hydrolysis (0.2 n HCl) it was possible to obtain extracts from nonsporulated cells of different strains of 5 species of the genus Bacillus which were composed mainly or solely of species-specific antigens. This was the result of experiments using the immunodiffusion technique after having prepared antisera from rabbits with the aid of HCl extracts. Cross-reacting antibodies were adsorbed easily. The species-specific reacting antisera revealed the existence of group- or strain-specific antigens. Therefore HCl extracts could become helpful in serotyping strains which belong to the taxa of the genus Bacillus.</p>","PeriodicalId":23838,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","volume":"245 4","pages":"512-9"},"PeriodicalIF":0.0000,"publicationDate":"1979-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
By acid hydrolysis (0.2 n HCl) it was possible to obtain extracts from nonsporulated cells of different strains of 5 species of the genus Bacillus which were composed mainly or solely of species-specific antigens. This was the result of experiments using the immunodiffusion technique after having prepared antisera from rabbits with the aid of HCl extracts. Cross-reacting antibodies were adsorbed easily. The species-specific reacting antisera revealed the existence of group- or strain-specific antigens. Therefore HCl extracts could become helpful in serotyping strains which belong to the taxa of the genus Bacillus.
通过酸水解(0.2 n HCl)，可以从芽孢杆菌属5种不同菌株的非孢子细胞中获得主要或完全由种特异性抗原组成的提取物。这是用盐酸提取物制备兔抗血清后用免疫扩散技术进行实验的结果。交叉反应抗体易于吸附。种特异性反应抗血清揭示了群体或菌株特异性抗原的存在。因此，HCl提取物可用于芽孢杆菌属菌株的血清分型。
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